FastQCFastQC Report
Wed 25 May 2016
SRR1780069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146628
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1497710.214283765720053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105327.182802738903893No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT98546.720408107592002No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG34322.3406170717734676No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT9280.6328941266333852RNA PCR Primer, Index 30 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC7510.5121804839457675No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC7360.5019505142264779No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC6650.4535286575551736No Hit
TATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG2570.17527348119049568No Hit
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC2530.17254548926535176No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATATCGTATGCCGT2320.1582235316583463No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT2270.15481354175191642No Hit
ATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1820.12412363259404753No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1730.11798565076247373No Hit
ATTATAAACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1680.11457566085604387No Hit
ATATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC1680.11457566085604387No Hit
TATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCT1610.10980167498704203No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC1550.10570968709932618No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCAGT1500.1022996971928963No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC150.002219091270.015351
CCGGGCT150.002222096869.9914858
CACGGAA150.002222096869.9914858
ATGTCGA150.002222096869.9914812
ACGGAAG150.002222096869.9914859
CATGTCG150.002222096869.9914811
CAATAGT150.002222096869.9914836
ATTATAC21050.064.360911
AGGGGGG32450.063.73645870
CGGCGTC252.377539E-456.031424
CTTTATA1000.056.0122832
CGTCTAC452.6844646E-854.43781748
CGAATGC452.6844646E-854.43781741
GCCGTCA1350.054.43781346
AGCTTGA1500.053.6601355
CAGCTTG1450.053.0969854
CGCTGGG200.006924628752.52943828
TCGCTGG200.006924628752.52943827
GCGTCAA200.006924628752.52943826
CCCATCG200.006924628752.52943823
Produced by FastQC (version 0.11.5)