FastQCFastQC Report
Wed 25 May 2016
SRR1780068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119456
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5377545.016575140637556No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG90347.562617197964104No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT8250.6906308598982053No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5650.47297749799089206No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5180.43363246718457005No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2870.24025582641307258No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2170.1816568443611037No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA150.00222011969.9991652
CGGCGAG150.00222011969.9991622
CGGCGAC150.00222011969.9991622
TTGTGCG353.6761776E-969.999162
AAGCGGG150.00222011969.9991630
TATGCGC150.00222011969.999163
CGCTGAC207.900082E-569.9991616
GTTGTGC150.00222011969.999161
CTTATGC207.900082E-569.999161
TCTCGGA150.00222011969.9991643
CACCGGA150.00222011969.9991626
TGCGCGT501.4551915E-1162.999255
TGTGCGC453.8016879E-1062.2214783
CCGGCGG308.334993E-658.33263821
TGCCGGC800.056.87431719
AAAAAGG11000.056.0227870
CTTGTGC252.3824845E-455.9993321
GCGCGTC509.695214E-1055.9993326
CTATACA252.3824845E-455.9993321
TTATGCA252.3824845E-455.9993322
Produced by FastQC (version 0.11.5)