##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780068_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 119456 Sequences flagged as poor quality 0 Sequence length 76 %GC 73 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69548620412537 32.0 27.0 32.0 22.0 32.0 2 29.31700375033485 32.0 32.0 32.0 22.0 32.0 3 29.08956435842486 32.0 32.0 32.0 13.0 32.0 4 28.35088233324404 32.0 27.0 32.0 13.0 32.0 5 29.23996283150281 32.0 32.0 32.0 13.0 32.0 6 33.07022669434771 37.0 37.0 37.0 13.0 37.0 7 32.878181087597106 37.0 32.0 37.0 13.0 37.0 8 32.991904969193676 37.0 37.0 37.0 13.0 37.0 9 33.1697193945888 37.0 37.0 37.0 13.0 37.0 10-11 32.9552638628449 37.0 34.5 37.0 13.0 37.0 12-13 32.72717988213233 37.0 32.0 37.0 13.0 37.0 14-15 33.43316367532815 37.0 34.5 37.0 20.0 37.0 16-17 33.7642604808465 37.0 37.0 37.0 20.0 37.0 18-19 33.242252377444416 37.0 34.5 37.0 17.5 37.0 20-21 33.71193577551567 37.0 37.0 37.0 20.0 37.0 22-23 33.80155873292258 37.0 37.0 37.0 20.0 37.0 24-25 33.034887322528796 37.0 34.5 37.0 13.0 37.0 26-27 33.691999564693276 37.0 37.0 37.0 20.0 37.0 28-29 33.486735701848374 37.0 37.0 37.0 17.5 37.0 30-31 33.02745780873292 37.0 34.5 37.0 17.5 37.0 32-33 33.349852665416556 37.0 37.0 37.0 17.5 37.0 34-35 33.44501741226895 37.0 37.0 37.0 22.0 37.0 36-37 34.242017981516206 37.0 37.0 37.0 27.0 37.0 38-39 34.392378783819986 37.0 37.0 37.0 27.0 37.0 40-41 33.80629687918564 37.0 37.0 37.0 20.0 37.0 42-43 33.508212228770425 37.0 37.0 37.0 22.0 37.0 44-45 33.34564609563354 37.0 37.0 37.0 17.5 37.0 46-47 33.772790818376635 37.0 37.0 37.0 20.0 37.0 48-49 34.37792576346102 37.0 37.0 37.0 27.0 37.0 50-51 34.69149728770426 37.0 37.0 37.0 32.0 37.0 52-53 33.56490674390571 37.0 37.0 37.0 17.5 37.0 54-55 33.798277189927674 37.0 37.0 37.0 20.0 37.0 56-57 33.62569063085989 37.0 37.0 37.0 20.0 37.0 58-59 33.14012690865256 37.0 37.0 37.0 13.0 37.0 60-61 32.905475656308596 37.0 34.5 37.0 13.0 37.0 62-63 32.496701714438785 37.0 32.0 37.0 13.0 37.0 64-65 32.35554095231717 37.0 32.0 37.0 13.0 37.0 66-67 32.50732068711493 37.0 32.0 37.0 13.0 37.0 68-69 32.72819280739351 37.0 32.0 37.0 13.0 37.0 70-71 32.66054865389767 37.0 32.0 37.0 13.0 37.0 72-73 32.572486940798285 37.0 32.0 37.0 13.0 37.0 74-75 32.14213183096705 37.0 32.0 37.0 13.0 37.0 76 33.3959616930083 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.17415830539192356 11101 2 -0.17931357355058353 11101 3 0.09743350166397846 11101 4 1.028628170792068 11101 5 0.33288239137344533 11101 6 -0.23438268083682345 11101 7 0.39005995222822065 11101 8 1.125230302893712 11101 9 0.7589512295414096 11101 10-11 -1.0590496737376505 11101 12-13 -0.23592085402332685 11101 14-15 -0.9017673585632622 11101 16-17 -0.2682501871948162 11101 18-19 0.3077940177301315 11101 20-21 -0.34726408147423626 11101 22-23 0.47024903235088544 11101 24-25 0.25806471476172277 11101 26-27 1.0832934340932 11101 28-29 -0.07001380885034081 11101 30-31 -0.8065511566952068 11101 32-33 0.8510360587076917 11101 34-35 0.5563306028029373 11101 36-37 0.9430867078001199 11101 38-39 0.032317727018522646 11101 40-41 -0.15406547176964125 11101 42-43 0.7831817366147504 11101 44-45 1.0388858510479437 11101 46-47 0.9143495451419668 11101 48-49 0.3260408805131192 11101 50-51 0.28189842819097066 11101 52-53 1.3182320189690415 11101 54-55 0.40094805580977066 11101 56-57 -0.014521849423431377 11101 58-59 1.0808509648712814 11101 60-61 0.6936940320020355 11101 62-63 -0.06275469195111327 11101 64-65 0.7050417153140884 11101 66-67 0.020287873186347838 11101 68-69 0.5758604353119878 11101 70-71 0.10953036434059271 11101 72-73 0.8180660662816308 11101 74-75 -0.6306263830582104 11101 76 -0.5331399704022886 11102 1 1.117327501985777 11102 2 1.5213528462939188 11102 3 -0.20115961199762822 11102 4 2.12951673058474 11102 5 0.4369179337651552 11102 6 1.6277054346759456 11102 7 2.20253681265028 11102 8 0.21075418293813897 11102 9 1.635293325061582 11102 10-11 2.0012057872027427 11102 12-13 1.4901065432369407 11102 14-15 1.525481808411925 11102 16-17 1.321809050124692 11102 18-19 1.9184012002551256 11102 20-21 1.4593816053084367 11102 22-23 1.4260987176526214 11102 24-25 1.262414955413341 11102 26-27 1.224167184555995 11102 28-29 1.0875204377249972 11102 30-31 2.333859802208906 11102 32-33 1.3200290242759962 11102 34-35 1.4072191625956094 11102 36-37 1.5419389847345926 11102 38-39 1.2849093597471324 11102 40-41 2.088159630044501 11102 42-43 1.35028651262364 11102 44-45 1.3671901827769375 11102 46-47 1.177215150473323 11102 48-49 1.3498283666293744 11102 50-51 1.2411727710269531 11102 52-53 1.268898438813551 11102 54-55 1.0416366896490885 11102 56-57 0.8380327599804929 11102 58-59 1.4140608871223037 11102 60-61 2.4079665236717958 11102 62-63 1.6830801840207457 11102 64-65 2.0903619670727025 11102 66-67 1.1438532193544262 11102 68-69 1.9909844634497134 11102 70-71 1.3055688685982787 11102 72-73 1.8647154553967766 11102 74-75 2.0768893518547884 11102 76 1.7262084190830862 11103 1 0.34844797968159824 11103 2 0.5694736752366651 11103 3 0.11267951690999567 11103 4 1.484276126440026 11103 5 0.32803138652244357 11103 6 0.07677463032048593 11103 7 0.4638645260327934 11103 8 0.88198705965047 11103 9 0.24751671810689402 11103 10-11 0.8820452673572916 11103 12-13 0.911515043412571 11103 14-15 1.024428067632158 11103 16-17 0.9587075397629121 11103 18-19 0.3937261036622175 11103 20-21 0.4470879628778164 11103 22-23 0.8598886719905252 11103 24-25 1.1263945830915958 11103 26-27 0.8892532400530087 11103 28-29 0.03896055012401689 11103 30-31 1.0396026894586399 11103 32-33 0.29403675170838994 11103 34-35 0.5119785584508918 11103 36-37 0.6307166454300557 11103 38-39 0.4585131532139428 11103 40-41 0.657091839387661 11103 42-43 1.185815139248156 11103 44-45 0.34831066047274817 11103 46-47 1.4302885610809781 11103 48-49 0.4036569581291971 11103 50-51 0.5882047344972676 11103 52-53 0.7657372164742426 11103 54-55 1.0024726573343727 11103 56-57 1.1784788435772668 11103 58-59 0.9937061277264476 11103 60-61 0.6479559862639945 11103 62-63 0.831042951846527 11103 64-65 0.7305094907818699 11103 66-67 0.9871970900955631 11103 68-69 0.7974474068989537 11103 70-71 0.22179647660670554 11103 72-73 1.8165068147223806 11103 74-75 1.1117506093187828 11103 76 1.1113506740883565 11104 1 -0.532614732706417 11104 2 -0.05085378846429123 11104 3 -0.5390041407977577 11104 4 0.7962934264091324 11104 5 0.6419022546101765 11104 6 0.2066788626343552 11104 7 -0.9568954368958451 11104 8 -0.10596474757844732 11104 9 -0.20197355066048317 11104 10-11 0.5242293884570728 11104 12-13 0.4146815556152248 11104 14-15 -0.4945710055837793 11104 16-17 0.2598351270350818 11104 18-19 0.679252825935933 11104 20-21 -0.413122108175628 11104 22-23 0.34382441496240546 11104 24-25 0.5067622074351235 11104 26-27 -0.7667554099315481 11104 28-29 0.2552873959689421 11104 30-31 1.178388602340931 11104 32-33 -0.5782215121161443 11104 34-35 0.676405496974219 11104 36-37 0.3232560337525783 11104 38-39 -0.2201244742188635 11104 40-41 0.2418153488099719 11104 42-43 -0.10341871926281243 11104 44-45 0.584717827602816 11104 46-47 0.46010011498891856 11104 48-49 0.24618090005073157 11104 50-51 -1.0222370325709988 11104 52-53 0.20271590044087873 11104 54-55 0.06441272529853137 11104 56-57 -0.32932158892196384 11104 58-59 0.20833386946262067 11104 60-61 0.045778043724602924 11104 62-63 0.44913068740413564 11104 64-65 1.3417235778995718 11104 66-67 0.7470055547233017 11104 68-69 0.8621678270410627 11104 70-71 1.2143822041321997 11104 72-73 0.9066365644906824 11104 74-75 0.40747195624494736 11104 76 0.9440661513821453 11105 1 -0.13552637929275946 11105 2 0.4637044444674352 11105 3 -0.008046978816501849 11105 4 -0.08623669407279522 11105 5 1.2521766856677417 11105 6 1.3748515533974057 11105 7 0.14869358586184944 11105 8 1.687542615206027 11105 9 1.0637560343462127 11105 10-11 -0.8118649890529639 11105 12-13 0.646023590421116 11105 14-15 0.2840434522475448 11105 16-17 -0.023130067074696115 11105 18-19 0.9209696934058087 11105 20-21 0.5245452278350768 11105 22-23 -0.4317779946761462 11105 24-25 0.4613304805274936 11105 26-27 0.764787428087196 11105 28-29 0.4091528578163306 11105 30-31 0.38789328774923604 11105 32-33 -0.21878376111212106 11105 34-35 0.0247990712714099 11105 36-37 0.046314936028345244 11105 38-39 -0.559649239948449 11105 40-41 0.6982243180201451 11105 42-43 0.6345330879661049 11105 44-45 -0.00371925405715956 11105 46-47 0.8501603559527737 11105 48-49 0.6798321718044136 11105 50-51 0.2201491789417176 11105 52-53 0.6765278147648388 11105 54-55 -0.04293332557161733 11105 56-57 0.6517694418678559 11105 58-59 -0.5420844705641557 11105 60-61 -0.30236452655652357 11105 62-63 0.4218549176584929 11105 64-65 -0.10389221861984055 11105 66-67 0.5347398251383026 11105 68-69 0.16763971459126026 11105 70-71 0.8403862201964571 11105 72-73 0.7043273275428916 11105 74-75 0.6165583016264762 11105 76 -0.3961279583902808 11106 1 0.4526770839107037 11106 2 -0.029260923497933078 11106 3 -0.1831912789608019 11106 4 -0.5959769538130537 11106 5 -0.4305433720523162 11106 6 -0.8940878405419852 11106 7 -0.5960755339072676 11106 8 -0.9621687845053728 11106 9 0.17558864617882364 11106 10-11 -0.2758801405681126 11106 12-13 0.6342090161065457 11106 14-15 0.08436812757221901 11106 16-17 0.08599691705229162 11106 18-19 -0.1431536832175695 11106 20-21 0.11175662754648386 11106 22-23 0.21919603129787646 11106 24-25 0.41452312122013524 11106 26-27 -0.4017890509892794 11106 28-29 -0.4855982244347601 11106 30-31 -0.08252518266922948 11106 32-33 0.10156255923419621 11106 34-35 0.44795924443157986 11106 36-37 1.2008964656098797 11106 38-39 0.021699966400760218 11106 40-41 -0.563770631474803 11106 42-43 0.2741434775765015 11106 44-45 0.6830592452213367 11106 46-47 0.3160694468618672 11106 48-49 0.84929465376689 11106 50-51 0.28733893363147445 11106 52-53 0.659843110580141 11106 54-55 0.07872973359144453 11106 56-57 0.37236828746670625 11106 58-59 0.6201630041833184 11106 60-61 0.14271123101924132 11106 62-63 -0.6840253132217384 11106 64-65 0.20653346680584406 11106 66-67 -0.012969410070937215 11106 68-69 0.38219599164754214 11106 70-71 0.10391435872458743 11106 72-73 1.4061671543827217 11106 74-75 1.2012444488126164 11106 76 2.443518506256183 11107 1 -0.40446577323215394 11107 2 -0.2630271572641689 11107 3 -0.4948795906491128 11107 4 -0.9726003304364319 11107 5 -0.39158233309127866 11107 6 -0.5434384898926368 11107 7 -0.1545170923488257 11107 8 -0.6634674858040768 11107 9 -0.3179178473276707 11107 10-11 0.42541856073058426 11107 12-13 -0.09306371116618806 11107 14-15 -0.6299175867134963 11107 16-17 0.0535293845847562 11107 18-19 0.1425606024967152 11107 20-21 0.02734104313088892 11107 22-23 0.06335187545372634 11107 24-25 -0.040022333325318016 11107 26-27 0.3644447152444812 11107 28-29 0.4234926846561464 11107 30-31 1.1187735186294674 11107 32-33 0.32883528650691574 11107 34-35 0.05185534832768468 11107 36-37 0.01907828379169274 11107 38-39 -0.010767566066775203 11107 40-41 -0.063770631474803 11107 42-43 0.6312863347193485 11107 44-45 0.32591638807848256 11107 46-47 0.8874980182904366 11107 48-49 0.310333614805856 11107 50-51 0.07305321934575915 11107 52-53 0.3351677859048152 11107 54-55 0.19561285047456778 11107 56-57 0.398342313440736 11107 58-59 0.32146170548202235 11107 60-61 0.01933460764261241 11107 62-63 0.5367539075574825 11107 64-65 0.109130869403252 11107 66-67 0.33118643408490556 11107 68-69 0.22635183580338492 11107 70-71 -0.18829343348320293 11107 72-73 0.6139593621749242 11107 74-75 0.889556137124309 11107 76 0.3785834413211191 11108 1 -0.5873054055099907 11108 2 -0.3391981772554118 11108 3 -0.3524602122746785 11108 4 0.23679701379237272 11108 5 0.1540181338575053 11108 6 0.8219489316745623 11108 7 -0.6510879372342799 11108 8 -0.2865522661922242 11108 9 -0.6522239906225806 11108 10-11 -0.24983315384697136 11108 12-13 -0.022437707955909048 11108 14-15 -0.52915879494347 11108 16-17 -1.1234733879236316 11108 18-19 -0.22158648636947476 11108 20-21 0.4080502215928803 11108 22-23 0.08086245476205534 11108 24-25 -0.32354779662606603 11108 26-27 0.15344223457908157 11108 28-29 0.7165030450822414 11108 30-31 0.8198533376868156 11108 32-33 0.36174058345716276 11108 34-35 -0.5585342620619258 11108 36-37 -1.070882317404859 11108 38-39 -0.09226472059764035 11108 40-41 5.807474833119386E-4 11108 42-43 -0.34580399068558165 11108 44-45 -0.09025171348288552 11108 46-47 0.01729518741344549 11108 48-49 0.25809371986933627 11108 50-51 0.1537478056583268 11108 52-53 -0.13659640496049974 11108 54-55 -0.5828053605279209 11108 56-57 -0.0824690100672143 11108 58-59 0.6834929326817942 11108 60-61 0.2034875333685804 11108 62-63 -0.25027367160492986 11108 64-65 0.49801165154246263 11108 66-67 -0.20222958809516456 11108 68-69 -0.5034745176184714 11108 70-71 -0.4597074127623486 11108 72-73 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52-53 0.25958337032039935 11109 54-55 -0.015988881127164234 11109 56-57 0.32232499742342213 11109 58-59 -0.5701400261197094 11109 60-61 0.22513547344347984 11109 62-63 0.05129936210293806 11109 64-65 0.9966633369357183 11109 66-67 -0.11998239708393044 11109 68-69 -0.42152695207539637 11109 70-71 -0.6879471131368788 11109 72-73 -0.5940060057904404 11109 74-75 -0.0992750317068598 11109 76 -0.612239069501392 11110 1 1.1471312783648955 11110 2 0.1855512913142796 11110 3 0.6785145827450592 11110 4 -0.7011015589376584 11110 5 -1.6671176086265547 11110 6 -0.6262745727287182 11110 7 0.2819518441201083 11110 8 0.35496003262344544 11110 9 1.353545824135999 11110 10-11 -0.05904967373765402 11110 12-13 0.24380887570639942 11110 14-15 0.523908317112415 11110 16-17 0.33985792091329614 11110 18-19 0.402388612324728 11110 20-21 0.15273591852577084 11110 22-23 0.7337625458644013 11110 24-25 0.7107674174644316 11110 26-27 -0.35589575509598603 11110 28-29 0.6326888938523609 11110 30-31 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22-23 -0.5290002168983676 21611 24-25 -0.7817250750280635 21611 26-27 -0.6449347941350254 21611 28-29 -0.8724829446528091 21611 30-31 -1.0071684406458203 21611 32-33 -0.8607590697541028 21611 34-35 -0.44742315095081153 21611 36-37 -0.13115419977412301 21611 38-39 -0.2031677584669609 21611 40-41 -0.20686827457244306 21611 42-43 0.0018170385833826685 21611 44-45 0.3936572891527135 21611 46-47 0.6510862818786975 21611 48-49 0.4228877273599707 21611 50-51 0.11289609252196442 21611 52-53 -0.17300922227219928 21611 54-55 -0.6779641897691491 21611 56-57 0.10701635544810983 21611 58-59 -0.8052017545147692 21611 60-61 -0.5346176129762696 21611 62-63 -0.3790710082674309 21611 64-65 -0.4850650581260112 21611 66-67 -1.1357848662197298 21611 68-69 -0.37016892738404295 21611 70-71 -0.28770019955663173 21611 72-73 -0.21079612924722824 21611 74-75 -0.3410034267685873 21611 76 -0.25569585962485064 21612 1 0.21566409689771504 21612 2 0.32679968256267244 21612 3 -0.27193586770539113 21612 4 0.723287115451015 21612 5 -0.23790267941162213 21612 6 -0.16681511326925857 21612 7 0.9602015223697862 21612 8 0.17960610726952098 21612 9 0.92883539942558 21612 10-11 0.3637302490422698 21612 12-13 0.9902696221671548 21612 14-15 0.545948214152304 21612 16-17 1.1812350122903794 21612 18-19 0.4775173124534291 21612 20-21 0.13069602148587478 21612 22-23 0.580126767228613 21612 24-25 0.4047828614798661 21612 26-27 0.32927155507132255 21612 28-29 0.1621290482925133 21612 30-31 -0.3303606805047252 21612 32-33 0.7038352865069228 21612 34-35 0.7509895474618844 21612 36-37 0.02448953920295338 21612 38-39 1.1050332997340888 21612 40-41 0.4908830481788726 21612 42-43 1.2238188022518202 21612 44-45 0.44766963483172617 21612 46-47 0.5346841654765839 21612 48-49 1.0836020130742554 21612 50-51 0.17749044878299003 21612 52-53 1.044583370320396 21612 54-55 0.43424921411092754 21612 56-57 0.6716107117091354 21612 58-59 1.2922409262612433 21612 60-61 0.8910878543958631 21612 62-63 1.1698707906743664 21612 64-65 0.618330003602388 21612 66-67 1.5535890314875047 21612 68-69 1.0218063812579317 21612 70-71 0.6796719344821653 21612 72-73 1.3114701846857457 21612 74-75 1.3695344921026589 21612 76 1.165379978117656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 12.0 12 43.0 13 91.0 14 186.0 15 353.0 16 659.0 17 970.0 18 1224.0 19 1275.0 20 1193.0 21 1171.0 22 1303.0 23 1462.0 24 1571.0 25 1823.0 26 1947.0 27 2041.0 28 2375.0 29 2678.0 30 3131.0 31 3979.0 32 5067.0 33 7830.0 34 13986.0 35 24937.0 36 38146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 63.98339137422984 9.067773908384677 5.858223948566836 21.090610768818642 2 56.385614787034555 8.487643986070186 25.769320921510847 9.357420305384409 3 56.02146396999732 10.025448700776856 24.685239753549425 9.267847575676399 4 56.08890377129463 17.219036457243313 15.718889958561801 10.973169812900254 5 55.29818510581301 15.229875435306726 24.573064559335656 4.8988748995446025 6 61.73621865974634 24.429282993595915 7.724247624628521 6.110250722029216 7 59.35490892043932 13.665282614519153 9.062751138494509 17.917057326547013 8 59.9899543761249 23.92449039387217 8.131932526892973 7.953622703109958 9 58.47927270291991 8.032246182694884 7.799524511117065 25.68895660326815 10-11 60.23431221537637 15.809586793463701 15.332005089740155 8.62409590141977 12-13 59.30970399142781 10.47080096437182 15.805401151888562 14.414093892311813 14-15 65.45841146530941 14.169233860166086 9.677203321725154 10.695151352799357 16-17 64.97162135012054 11.30834784355746 9.29672850254487 14.423302303777122 18-19 59.272281309776446 10.500981537057733 14.855741259297394 15.370995893868427 20-21 65.21131299940144 10.557069368928177 10.344019321002381 13.88759831066799 22-23 65.06077551567104 10.103301634074471 9.075726627377446 15.760196222877044 24-25 61.050604830270814 15.626805073040057 9.034782972667532 14.287807124021597 26-27 65.33018571769404 15.283934184696394 9.606924754406453 9.778955343203117 28-29 65.63168403032117 11.47466190055628 10.085848586700376 12.807805482422177 30-31 60.37654504434743 15.410888664721714 15.010317736041756 9.2022485548891 32-33 65.00502302218501 16.533696107157805 10.21515278359146 8.246128087065719 34-35 60.815205789652126 20.828014013703964 10.422796964534614 7.933983232109297 36-37 65.31162362395881 11.575907245406219 15.175170566322047 7.937298564312921 38-39 65.88199839271364 10.143902357353335 16.012590409858024 7.961508840075007 40-41 65.24199590639114 15.113285811752592 11.314349815201604 8.330368466654669 42-43 60.983634004436816 15.299066594114938 15.243397095140429 8.473902306307815 44-45 60.16441200107152 9.991545004018217 16.315212295740693 13.52883069916957 46-47 64.50866229401153 9.603656622395976 11.701526522650884 14.186154560941613 48-49 66.21601259040986 9.315563889633003 15.833444950442004 8.634978569515136 50-51 70.39483322241337 9.51693308386805 11.580463017609068 8.507770676109514 52-53 60.82374115775815 10.007534217906326 15.579088359633333 13.58963626470219 54-55 64.72410228076563 9.859738563732938 10.827462946452863 14.588696209048557 56-57 64.44560713239295 10.102549077058306 10.475074295759908 14.976769494788833 58-59 60.43974902577969 14.972227919417017 15.326756212616518 9.261266842186775 60-61 59.150897196026975 10.904897639706837 16.3684384561615 13.575766708104691 62-63 59.27361463988381 15.559480806499545 15.471162660259763 9.695741893356885 64-65 59.223386310268765 15.832388064074607 16.060926963823047 8.883298661833585 66-67 59.36193278612358 21.703661226659523 9.99836757928751 8.936038407929379 68-69 59.556148993968414 22.159483317916028 9.23029270559083 9.054074982524726 70-71 59.96835641724324 22.035402304623897 9.013816860671966 8.982424417460894 72-73 60.817807766201916 21.198206833621448 8.99094626779458 8.993039132382057 74-75 65.82546054256329 16.61008861337028 8.868034306810713 8.696416537255706 76 72.27696248733811 10.414974927377294 8.7833708655287 8.524691719755888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.5 11 3.0 12 4.0 13 7.5 14 12.0 15 16.5 16 29.0 17 38.0 18 42.0 19 68.0 20 110.5 21 131.0 22 139.0 23 174.0 24 224.5 25 248.0 26 311.0 27 399.5 28 501.0 29 577.0 30 659.0 31 766.0 32 944.0 33 1097.0 34 1178.5 35 1393.0 36 1557.5 37 1589.0 38 1773.0 39 2071.5 40 2235.0 41 2606.0 42 2928.0 43 3992.5 44 7796.5 45 6957.0 46 3378.0 47 3168.5 48 2592.0 49 2096.0 50 1967.0 51 1886.5 52 1623.5 53 1341.0 54 1241.0 55 1189.5 56 1024.5 57 844.5 58 778.0 59 717.5 60 621.5 61 520.5 62 455.0 63 446.5 64 413.0 65 359.0 66 322.5 67 315.0 68 281.0 69 267.5 70 244.5 71 201.0 72 219.0 73 223.0 74 202.5 75 196.0 76 175.0 77 178.0 78 202.5 79 203.0 80 194.5 81 187.0 82 182.5 83 177.0 84 177.0 85 200.0 86 244.5 87 266.0 88 263.5 89 279.0 90 310.0 91 377.5 92 432.0 93 456.0 94 516.5 95 654.5 96 756.0 97 929.0 98 1605.0 99 28402.0 100 54696.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 8.371283150281276E-4 5 0.0 6 8.371283150281276E-4 7 0.0 8 8.371283150281276E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.185641575140638E-4 20-21 4.185641575140638E-4 22-23 0.0 24-25 8.371283150281276E-4 26-27 4.185641575140638E-4 28-29 0.0012556924725421912 30-31 0.0012556924725421912 32-33 0.005022769890168765 34-35 0.0012556924725421912 36-37 8.371283150281276E-4 38-39 0.0 40-41 0.0012556924725421912 42-43 8.371283150281276E-4 44-45 0.0 46-47 0.0012556924725421912 48-49 0.0 50-51 4.185641575140638E-4 52-53 8.371283150281276E-4 54-55 4.185641575140638E-4 56-57 8.371283150281276E-4 58-59 0.0012556924725421912 60-61 4.185641575140638E-4 62-63 0.0012556924725421912 64-65 0.0012556924725421912 66-67 0.0012556924725421912 68-69 0.0012556924725421912 70-71 4.185641575140638E-4 72-73 0.0020928207875703184 74-75 0.003767077417626574 76 0.0025113849450843825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 119456.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.043430216983662 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8585358303668 19.973881596571122 2 10.59897848630243 5.732654701312617 3 6.630552546045504 5.379386552370748 4 3.767218696796162 4.075140637556925 5 2.355672496517567 3.1852732386820253 6 1.2072434607645874 1.9588802571658186 7 0.6129082185420214 1.1602598446289847 8 0.28478563689831293 0.6161264398607018 9 0.1547748026621266 0.37670774176265737 >10 0.45503791982665226 1.870981784087865 >50 0.04333694474539545 0.7952718992767212 >100 0.015477480266212661 0.6990021430484865 >500 0.009286488159727596 1.5972408250736672 >1k 0.0 0.0 >5k 0.003095496053242532 7.562617197964104 >10k+ 0.003095496053242532 45.016575140637556 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 53775 45.016575140637556 No Hit CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG 9034 7.562617197964104 No Hit TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT 825 0.6906308598982053 No Hit CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG 565 0.47297749799089206 No Hit ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC 518 0.43363246718457005 No Hit TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG 287 0.24025582641307258 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 217 0.1816568443611037 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004185641575140638 0.0 2 0.0 0.0 0.0 0.005859898205196893 0.0 3 0.0 0.0 0.0 0.005859898205196893 0.0 4 0.0 0.0 0.0 0.014231181355478168 0.0 5 0.0 0.0 0.0 0.020928207875703188 0.0 6 0.0 0.0 0.0 0.022602464505759444 0.0 7 0.0 0.0 0.0 0.023439592820787572 0.0 8 0.0 0.0 0.0 0.023439592820787572 0.0 9 0.0 0.0 0.0 0.025113849450843825 0.0 10 0.0 0.0 0.0 0.025113849450843825 0.0 11 0.0 0.0 0.0 0.028462362710956337 0.0 12 0.0 0.0 0.0 0.03013661934101259 0.0 13 0.0 0.0 0.0 0.03013661934101259 0.0 14 0.0 0.0 0.0 0.03013661934101259 0.0 15 0.0 0.0 0.0 0.03013661934101259 0.0 16 0.0 0.0 0.0 0.03013661934101259 0.0 17 0.0 0.0 0.0 0.03013661934101259 0.0 18 0.0 0.0 0.0 0.030973747656040718 0.0 19 0.0 0.0 0.0 0.032648004286096974 0.0 20 0.0 0.0 0.0 0.03683364586123761 0.0 21 0.0 0.0 0.0 0.04101928743637825 0.0 22 0.0 0.0 0.0 0.05022769890168765 0.0 23 0.0 0.0 0.0 0.051901955531743905 0.0 24 0.0 0.0 0.0 0.05943611036699705 0.0 25 0.0 0.0 0.0 0.05943611036699705 0.0 26 0.0 0.0 0.0 0.06027323868202518 0.0 27 0.0 0.0 0.0 0.07031877846236272 0.0 28 0.0 0.0 0.0 0.0761786766675596 0.0 29 0.0 0.0 0.0 0.09459549959817841 0.0 30 0.0 0.0 0.0 0.11384945084382535 0.0 31 0.0 0.0 0.0 0.14147468523975354 0.0 32 0.0 0.0 0.0 0.164077149745513 0.0 33 0.0 0.0 0.0 0.18751674256630058 0.0 34 0.0 0.0 0.0 0.2469528529332976 0.0 35 0.0 0.0 0.0 0.3005290650950978 0.0 36 0.0 0.0 0.0 0.41856415751406373 0.0 37 0.0 0.0 0.0 0.6420774176265738 0.0 38 0.0 0.0 0.0 0.8429882132333244 0.0 39 0.0 0.0 0.0 1.1150549156174658 0.0 40 0.0 0.0 0.0 1.3996785427270293 0.0 41 0.0 0.0 0.0 1.745412536833646 0.0 42 0.0 0.0 0.0 2.1355143316367533 0.0 43 0.0 0.0 0.0 2.539847307795339 0.0 44 0.0 0.0 0.0 2.9065095097776585 0.0 45 0.0 0.0 0.0 3.2154098580230377 0.0 46 0.0 0.0 0.0 3.5510983123493167 0.0 47 0.0 0.0 0.0 3.9227832842218056 0.0 48 0.0 0.0 0.0 4.170573265470131 0.0 49 0.0 0.0 0.0 4.453522635949638 0.0 50 0.0 0.0 0.0 4.74316903294937 0.0 51 0.0 0.0 0.0 5.036163943209215 0.0 52 0.0 0.0 0.0 5.305719260648273 0.0 53 0.0 0.0 0.0 5.590342887757836 0.0 54 0.0 0.0 0.0 5.857386820251808 0.0 55 0.0 0.0 0.0 6.132802035896062 0.0 56 0.0 0.0 0.0 6.448399410661667 0.0 57 0.0 0.0 0.0 6.707072060005357 0.0 58 0.0 0.0 0.0 6.948165014733458 0.0 59 0.0 0.0 0.0 7.1984663809268685 0.0 60 0.0 0.0 0.0 7.425328154299491 0.0 61 0.0 0.0 0.0 7.685675060273239 0.0 62 0.0 0.0 0.0 7.9250937583712835 0.0 63 0.0 0.0 0.0 8.177906509509778 0.0 64 0.0 0.0 0.0 8.453321725154032 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCGA 15 0.002220119 69.99916 52 CGGCGAG 15 0.002220119 69.99916 22 CGGCGAC 15 0.002220119 69.99916 22 TTGTGCG 35 3.6761776E-9 69.99916 2 AAGCGGG 15 0.002220119 69.99916 30 TATGCGC 15 0.002220119 69.99916 3 CGCTGAC 20 7.900082E-5 69.99916 16 GTTGTGC 15 0.002220119 69.99916 1 CTTATGC 20 7.900082E-5 69.99916 1 TCTCGGA 15 0.002220119 69.99916 43 CACCGGA 15 0.002220119 69.99916 26 TGCGCGT 50 1.4551915E-11 62.99925 5 TGTGCGC 45 3.8016879E-10 62.221478 3 CCGGCGG 30 8.334993E-6 58.332638 21 TGCCGGC 80 0.0 56.874317 19 AAAAAGG 1100 0.0 56.02278 70 CTTGTGC 25 2.3824845E-4 55.999332 1 GCGCGTC 50 9.695214E-10 55.999332 6 CTATACA 25 2.3824845E-4 55.999332 1 TTATGCA 25 2.3824845E-4 55.999332 2 >>END_MODULE