FastQCFastQC Report
Wed 25 May 2016
SRR1780068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119456
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2471120.68627779266006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1353611.331368872220734No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT100428.406442539512456No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG39013.2656375569247253No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT10790.9032614519153496TruSeq Adapter, Index 6 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC7160.5993838735601392No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC6670.558364586123761No Hit
TATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG5250.439492365389767No Hit
ATTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT4960.41521564425395124No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT4590.3842418965979105No Hit
ATTATAAACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT3910.32731717117599785No Hit
ATATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC3120.2611840342887758No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC2790.23355879989284756No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT2770.23188454326279132No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGA2560.21430484864720067No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT2510.21011920707206No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTAGTATGCCGT2370.19839941066166622No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT2360.19756228234663809No Hit
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC2330.1950508974015537No Hit
ATTATACACATCTCCGAGACCACGAGACACCACAACATCTCGTATGCCGT2290.1917023841414412No Hit
ATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC2120.17747120278596304No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCACGTATGCCGT1670.1398004286096973No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCAGT1630.13645191534958478No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC1610.13477765871952851No Hit
TTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1580.13226627377444417No Hit
ATTATACACATCTCCGAGCCAACGAGACACCACAACATCTCGTATGCCGT1560.1305920171443879No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT1460.1222207339941066No Hit
ATTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1370.11468657915885347No Hit
CTATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC1350.11301232252879721No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT1350.11301232252879721No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT1270.1063152960085722No Hit
ATTATACACATATCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT1260.10547816769354407No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGAATGCCGT1260.10547816769354407No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATATCGTATGCCGT1210.10129252611840343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGAG800.069.9962412
AGCCGTA150.002220487569.9962345
AGATCTG150.002220487569.9962370
GACGTCA455.456968E-1269.9962346
AAGACGA150.002220487569.9962344
AGACGCA150.002220487569.9962325
CCGTAAT455.456968E-1269.9962347
AGTCTTA150.002220487569.9962348
CGTAATC353.6779966E-969.9962348
CGTAATA150.002220487569.9962348
CGTAAGA301.0168151E-769.9962341
GTAAGAC353.6779966E-969.9962342
GACGACA150.002220487569.9962346
CGTCATA252.8263312E-669.9962348
AGGTGAC150.002220487569.996237
GCCGTAA455.456968E-1269.9962346
TCGAAAG150.002220487569.9962340
ACAAGGT150.002220487569.9962318
ACGACAT150.002220487569.9962347
TGCCGAA150.002220487569.9962345
Produced by FastQC (version 0.11.5)