FastQCFastQC Report
Wed 25 May 2016
SRR1780067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245285
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5984424.3977414028579No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG93913.828607538169884No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT8830.3599894000856147No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG6240.2543979452473653No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4980.20302912938010886No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2600.10599914385306887No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGA150.00222389169.991442
CAGGTAT252.3887609E-455.9931451
TCGCCGT13050.052.8395652
GCCGTAT13150.052.69317254
ATCTCGG13250.052.57032442
TAGATCT13450.052.55862439
GGTAATG200.006943308352.50428448
GAGCGTC200.006948910752.4935828
GATGCGC200.006948910752.493583
TGAGCGT200.006948910752.4935827
AGAGCTC200.006948910752.493588
GATCTCG13300.052.37269241
GTCGCCG13250.052.30615251
CGTATCA13250.052.0419856
TCGGTGG13600.051.98953245
AGATCTC13400.051.98185340
GTAGATC13400.051.98185338
TGGTCGC13350.051.91434549
CTCGGTG13600.051.7321644
CGGTGGT13600.051.7321646
Produced by FastQC (version 0.11.5)