FastQCFastQC Report
Wed 25 May 2016
SRR1780067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245285
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3089712.596367490877958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139095.6705465071243655No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT90583.692847096234992No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG40421.6478789979004018No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT11610.47332694620543453Illumina PCR Primer Index 10 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC10150.41380435004178806No Hit
ATTATAAACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT8180.33348961412234746No Hit
TATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG8050.32818965692969404No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC7130.2906822675663004No Hit
ATATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC6240.2543979452473653No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGACGT5430.22137513504698614No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAGT2900.11822981429765375No Hit
ATTATACACATCTCCGAGACCACGAGACTTAAACTTATCTCGTATGCCGT2800.11415292414945878No Hit
ATTATACACATCTCCGAGCCAACGAGACTTAAACTTATCTCGTATGCCGT2770.1129298571050003No Hit
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC2730.11129910104572233No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC2650.10803758892716636No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGA2630.10722221089752737No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATATCGTATGCCGT2610.10640683286788838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGC150.002221630770.00938464
ATTCCGT150.002223428669.9951144
AAGCCGT301.0214171E-769.9951144
CTGCAAG207.918945E-569.9951154
TCGTATT150.002223428669.9951140
GATCATA150.00222522869.980831
ATTCACG150.00222522869.980831
AGGGGGG47500.065.8690870
ATGACGT1250.064.395544
TAACTCG550.063.63191636
ATTATAC44000.063.3008461
AACTCGT453.8380676E-1062.2178737
CCGACTT1350.062.2051947
CGTATGA1450.060.34060741
GTATGAC1450.060.34060742
TGCCGAC1650.057.26872345
TGACGTC1350.057.03304745
TTATCTA252.3881416E-455.99608235
CGACTTC1500.055.9846748
GTCTTCA950.055.24802849
Produced by FastQC (version 0.11.5)