FastQCFastQC Report
Wed 25 May 2016
SRR1780066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93093
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4272745.897113639049124No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG21182.2751442106280817No Hit
TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT2520.27069704489059326No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC2330.2502873470615406No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG1590.1707969449904934No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA150.002217584570.0069925
GGCAACG150.002217584570.0069940
CATGTAA150.002217584570.0069937
CGTCCTG150.002217584570.0069935
CTTAGTG150.002217584570.0069916
ATCCCAA150.002222320369.969376
GCCCGAA150.002222320369.969377
TGATAAC200.00692932852.5052423
GATAACC200.00692932852.5052424
GTGATTC200.00692932852.5052449
AAGGTGC200.00692932852.5052415
CCGTCCT200.00692932852.5052434
TGATTCC200.00692932852.5052450
GCTTAGT200.00692932852.5052415
ACTGTTG200.00692932852.5052444
CTGGCCT200.00692932852.5052425
AGCCCGA200.00694408452.4770286
GTGATTG200.00694408452.4770288
GAATACT200.00694408452.4770284
TACTGGT200.00694408452.4770287
Produced by FastQC (version 0.11.5)