FastQCFastQC Report
Wed 25 May 2016
SRR1780066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93093
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2925431.42448948900562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35163.7768682929973254No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG31173.3482646385872195No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC22762.444866960995993No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT7300.7841620744846551TruSeq Adapter, Index 11 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT6480.6960781154329542No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC6300.6767426122264832No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4310.462977882332721No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC2980.3201099975293524No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT2250.24169379008088684No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT2150.23095184385506967No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1590.1707969449904934No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1460.15683241489693103No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1380.14823885791627728No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT1290.1385711063130418RNA PCR Primer, Index 11 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1220.13105174395496977No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC1180.12675496546464288No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT1120.12030979772915257No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT1060.11386462999366226No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1050.11279043537108054No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT1040.11171624074849881No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTC150.002214547570.0311830
GTAATCT150.002219276769.9935549
ATATCCG252.8220566E-669.9935510
GCACAAC150.002219276769.993556
CACAGAT150.002219276769.993555
ACATATC252.8220566E-669.993558
GCCGTAA252.8220566E-669.9935546
GCTCCTG150.002219276769.9935546
CTATACA700.069.9935461
CGTAATC207.894049E-569.99354648
AGGGGGG34450.065.9300670
GCTATAA501.4551915E-1162.99419432
TGACGTC453.783498E-1062.2164945
ATAACTC401.0537406E-861.24435435
GTATGAC601.8189894E-1258.3279642
TATCCGA308.324518E-658.3279611
CTATAAC553.45608E-1157.26745233
ATGACGT553.45608E-1157.26745244
CAGGGGA252.3743582E-456.02494421
AGCCGTC509.658834E-1055.9948445
Produced by FastQC (version 0.11.5)