FastQCFastQC Report
Wed 25 May 2016
SRR1780065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536020
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG517949.662699153016678No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG22870.42666318420954447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGA200.006950866452.4983861
ATTCGCG200.006950866452.49838611
TGACGTA200.006950866452.4983865
ACGTACG200.006950866452.4983868
GTGCGAC305.877469E-446.66523411
GCCGTAT4350.044.25564254
TCGCCGT4400.043.74865752
TGGTCGC4500.042.77646649
CGGTGGT4900.039.28450846
CGCCGTA5050.038.81069653
TCTCGGT5000.037.79884343
TCGGTGG5200.037.01809745
CTCGGTG5150.036.69790644
CGTATCA5300.036.3230356
GTCGCCG5400.036.2951851
ATCTCGG5350.035.3260242
TAGATCT5350.035.3260239
AACGATA400.002417542735.0021914
AGATCTC5400.034.99892440
GGTCTAA400.002418649434.9989241
Produced by FastQC (version 0.11.5)