FastQCFastQC Report
Wed 25 May 2016
SRR1780065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536020
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT344906.434461400694004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40200.7499720159695533No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG37980.7085556509085482No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC37200.6940039550763031No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT9480.1768590724226708TruSeq Adapter, Index 9 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT8870.16547890004104324No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC6700.12499533599492556No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT6170.11510764523711803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA150.00222445569.9984167
ATCGGAT550.063.63492632
AGGGGGG42950.061.11619670
CGGATAT700.055.00388334
AACGGTT200.006948082752.50370864
CGACGCA200.006950646652.4988160
ATCGTAA200.006950646652.498816
CGACTTA200.006953211552.4939111
CCGTCTT48500.052.31352247
AAGGGGG50050.052.30650769
CGTCTTC49500.052.1099348
TATGCCG49900.052.03805543
TCGTATG51750.051.8732240
CGTATGC50900.051.7768241
TCTCGTA51450.051.76753238
CTCGTAT51050.051.76180639
GCCGTCT49500.051.7515746
CTTGAAA51000.051.67529757
GTATGCC50500.051.56318742
ATGCCGT50250.051.5363144