FastQCFastQC Report
Wed 25 May 2016
SRR1780063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99737
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4786047.98620371577248No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG18541.8588888777484787No Hit
TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT2280.22860122121178703No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC2120.21255903024955636No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG1190.11931379528159057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC150.002214960570.0316115
GAGTGTT150.002219374569.996497
ACCGGGG150.002219374569.996498
AAGAGTG150.002219374569.996497
GACCGGG150.002219374569.996497
TAGCGCA150.002219374569.996494
CAACCAT150.002219374569.996495
CTGCGTC150.002219374569.9964924
GGGACGA150.002219374569.9964912
GGGCGTA150.002219374569.9964925
CGCTAGG150.002219374569.9964945
GGACGAG150.002219374569.9964913
GGGCCGT150.002219374569.9964924
GGACCGG150.002219374569.996496
AGATGTA150.002219374569.9964911
TCGGCGC252.3810146E-455.9971936
GTGAAAT252.3810146E-455.9971940
CACGCAC200.00692114552.5237116
CTCTTGT200.00692114552.5237117
GAGACGC200.00692114552.5237117
Produced by FastQC (version 0.11.5)