FastQCFastQC Report
Wed 25 May 2016
SRR1780063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99737
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3141731.499844591275057Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG35843.5934507755396696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32583.2665911346842194No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC26272.6339272286112476No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT8930.8953547830794991Illumina PCR Primer Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT6860.6878089375056399Illumina PCR Primer Index 6 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC6510.6527166447757603No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT5830.58453733318628Illumina PCR Primer Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC3630.3639572074556082Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT2400.24063286443346002TruSeq Adapter, Index 6 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT1970.1975194762224651No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT1630.16342982042772491No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT1630.16342982042772491No Hit
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC1520.15240081414119133Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGACAATATCTCGTATGCCGT1490.14939290333577307No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT1400.14036917091951834Illumina PCR Primer Index 6 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG1320.132348075438403No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG1280.12833752769784534No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT1270.1273348907627059Illumina PCR Primer Index 6 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC1190.11931379528159057Illumina PCR Primer Index 6 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC1100.11029006286533583No Hit
CAAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT1090.10928742593019641Illumina PCR Primer Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTA150.00220979270.0728329
GATGACC150.002214200370.0376464
CGTATTC252.8213835E-670.0025148
GGGTAAT150.00221861570.002513
GCCGGGT150.00221861570.002513
CGTCTAC301.0144504E-770.002548
CCATGTT150.00221861570.002514
TACGAGA150.00221861570.002513
CGACTTA150.00221861570.002548
GCTTTCA150.00221861570.002555
ATCTCGA252.829811E-669.967437
GTCCTAC150.002223036369.967441
GACGTCA150.002223036369.967446
CCGTCTA301.0179974E-769.967447
AATATAC150.002223036369.967439
ACGTCAT150.002223036369.967447
TAGATCC150.002223036369.96744
CGTAAGA150.002223036369.967441
ACGTATG207.911442E-569.967440
CACGTAT252.829811E-669.967439
Produced by FastQC (version 0.11.5)