FastQCFastQC Report
Wed 25 May 2016
SRR1780062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322105
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4704214.604554415485635No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG20370.6324024774530045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC150.002222898970.0043550
TGGTACG150.002222898970.0043557
CAACCGG150.002224268269.99348423
TATGCGC150.002224268269.9934843
AACGGTA150.002224268269.9934842
TCCACGT150.002224268269.9934848
GGGTCTA352.971119E-759.9944081
GCGCGTC308.371082E-658.32796
GTTGTGC252.3895447E-455.994791
ACTTTTG252.3895447E-455.9947930
GATACGT200.006945810752.5032649
GGTACGC200.006945810752.5032658
GGTCACT200.006945810752.5032651
CCGCTTG200.006945810752.5032642
CGAGGAC200.006945810752.5032614
CAAGCTT200.006945810752.5032646
CCGCGAG200.006945810752.5032669
ACCCGCT200.006945810752.5032640
CGGCCAT200.00695007752.4951127
TGCGCGT407.47219E-752.495115
Produced by FastQC (version 0.11.5)