FastQCFastQC Report
Wed 25 May 2016
SRR1780062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322105
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT309299.602148367768274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37331.158938855342202No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC33741.0474845159187223No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG31700.9841511308424271No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT9200.28562114838329117No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT7310.22694462985672373RNA PCR Primer, Index 37 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6690.20769624811784976No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC5290.16423216032039242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA252.8353406E-670.00419618
GCGCTAT252.8353406E-670.00419619
CAGACGT150.002222918770.0041966
GACGTAT150.002224288269.99332468
AGGGGGG39350.063.41270
AAGCCGT750.060.6608844
AACTCGT352.9711555E-759.99427437
CCCGACT252.387738E-456.00335715
GCCTTTA252.3895707E-455.994661
CGTCTTC44250.055.045648
CCGTCTT43900.055.00614547
GCCGTCT44350.054.92148246
GTATGAC1150.054.77738642
CGTATGC46050.054.48991841
TCTTCTG44850.054.46526350
CGTATGA1350.054.43925541
TATGCCG45950.054.3800243
CTCGTAT47050.054.37313539
CTTGAAA45900.054.3714557
AAGGGGG45700.054.37118569