FastQCFastQC Report
Wed 25 May 2016
SRR1780061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60770
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4444573.13641599473424No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG21223.4918545334869178No Hit
TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT2140.35214744117163077No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC2020.33240085568537103No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG1870.30771762382754647No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG820.1349350008227744No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690.11354286654599308No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610.10037847622181997No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG150.002214747270.029
ATAGGTG150.002214747270.032
TCATGCC150.002214747270.018
AAGGCAT150.002214747270.027
ATAGAGT150.002214747270.042
CATAGGT150.002214747270.031
AGGCATA150.002214747270.028
GCATAGG150.002214747270.030
TAGGTGG150.002214747270.045
CAGCCGA252.3729929E-455.99999618
AAAAAGG1950.053.84615370
TAAAAAA3750.053.264
ACGCTGT200.006920541652.557
ATGATGC200.006920541652.520
CTAGGTG200.006920541652.544
GTGACCT200.006920541652.54
TAGAGTT200.006920541652.543
AGCCGAC200.006920541652.519
GAAGGCA200.006920541652.526
GTCGCCG3700.052.02702751
Produced by FastQC (version 0.11.5)