##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780061_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60770 Sequences flagged as poor quality 0 Sequence length 76 %GC 88 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.403505018923813 32.0 27.0 32.0 27.0 32.0 2 30.077702813888433 32.0 32.0 32.0 27.0 32.0 3 30.143508310021392 32.0 32.0 32.0 27.0 32.0 4 29.763946025999672 32.0 32.0 32.0 22.0 32.0 5 30.222198453184138 32.0 32.0 32.0 27.0 32.0 6 34.59850255060063 37.0 37.0 37.0 27.0 37.0 7 34.24592726674346 37.0 37.0 37.0 27.0 37.0 8 34.59091657067632 37.0 37.0 37.0 27.0 37.0 9 34.68247490538094 37.0 37.0 37.0 32.0 37.0 10-11 34.54800065821952 37.0 37.0 37.0 27.0 37.0 12-13 34.3164061214415 37.0 37.0 37.0 27.0 37.0 14-15 34.822988316603585 37.0 37.0 37.0 29.5 37.0 16-17 34.95330755306895 37.0 37.0 37.0 29.5 37.0 18-19 34.51488398881027 37.0 37.0 37.0 29.5 37.0 20-21 34.940389995063356 37.0 37.0 37.0 29.5 37.0 22-23 34.98129011025176 37.0 37.0 37.0 29.5 37.0 24-25 34.71523778180023 37.0 37.0 37.0 32.0 37.0 26-27 35.06940102024025 37.0 37.0 37.0 32.0 37.0 28-29 34.921309856837254 37.0 37.0 37.0 32.0 37.0 30-31 34.85416323844002 37.0 37.0 37.0 32.0 37.0 32-33 34.893779825571826 37.0 37.0 37.0 32.0 37.0 34-35 34.7474658548626 37.0 37.0 37.0 32.0 37.0 36-37 35.36409412539082 37.0 37.0 37.0 32.0 37.0 38-39 35.49184630574297 37.0 37.0 37.0 34.5 37.0 40-41 35.17539081783775 37.0 37.0 37.0 32.0 37.0 42-43 35.11027645219681 37.0 37.0 37.0 32.0 37.0 44-45 34.96880862267566 37.0 37.0 37.0 32.0 37.0 46-47 35.1187839394438 37.0 37.0 37.0 32.0 37.0 48-49 35.474008556853704 37.0 37.0 37.0 34.5 37.0 50-51 35.63477867368768 37.0 37.0 37.0 37.0 37.0 52-53 35.09928418627612 37.0 37.0 37.0 32.0 37.0 54-55 35.22950468981405 37.0 37.0 37.0 32.0 37.0 56-57 35.11849596840546 37.0 37.0 37.0 32.0 37.0 58-59 34.87344907026494 37.0 37.0 37.0 32.0 37.0 60-61 34.75259173934507 37.0 37.0 37.0 29.5 37.0 62-63 34.53223630080632 37.0 37.0 37.0 27.0 37.0 64-65 34.36994405134112 37.0 37.0 37.0 27.0 37.0 66-67 34.54405134112226 37.0 37.0 37.0 27.0 37.0 68-69 34.629685700181014 37.0 37.0 37.0 27.0 37.0 70-71 34.65295376007899 37.0 37.0 37.0 27.0 37.0 72-73 34.85742965278921 37.0 37.0 37.0 32.0 37.0 74-75 34.67661675168669 37.0 37.0 37.0 29.5 37.0 76 34.71308211288465 37.0 37.0 37.0 32.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.4684154648221117 11101 2 -0.3813610532451541 11101 3 -0.9568683484156892 11101 4 -0.6705361892332604 11101 5 -0.28205551634713544 11101 6 -0.727584025036272 11101 7 -1.1833742846190418 11101 8 -0.38773910693449665 11101 9 -0.9803656347351009 11101 10-11 0.01911901342568001 11101 12-13 -0.06674423432640708 11101 14-15 0.72965262281825 11101 16-17 -0.10931620037197831 11101 18-19 1.0320195031584447 11101 20-21 0.23553253011378672 11101 22-23 0.007001774134700156 11101 24-25 0.18581924176906028 11101 26-27 0.2637327056763823 11101 28-29 -0.23014439894571836 11101 30-31 0.6431460630305779 11101 32-33 0.5933125807909647 11101 34-35 -0.4913402426926581 11101 36-37 -0.2942921163785428 11101 38-39 -0.31959610371256275 11101 40-41 0.28469577336844765 11101 42-43 0.6828349040566835 11101 44-45 0.872034952502986 11101 46-47 -0.33118482877622313 11101 48-49 0.6175879284447348 11101 50-51 -0.46514098545817006 11101 52-53 -0.17441074234950094 11101 54-55 1.2260482949276152 11101 56-57 0.05519976878269972 11101 58-59 0.12766302671204244 11101 60-61 0.9830230035193281 11101 62-63 -0.0311662174625269 11101 64-65 0.5144006378258297 11101 66-67 0.4340701950417838 11101 68-69 1.4976202395254958 11101 70-71 0.66041641175471 11101 72-73 -0.33058091325705163 11101 74-75 1.3929846649704274 11101 76 1.1369212084333356 11102 1 1.6079852142950344 11102 2 0.6780616971792952 11102 3 -0.22936410393351636 11102 4 1.4754739975239524 11102 5 0.5923078113268865 11102 6 0.6119745488177202 11102 7 1.8251146797273137 11102 8 0.2608177691266249 11102 9 0.7573253669626894 11102 10-11 -0.39768913598008737 11102 12-13 0.7541386179656158 11102 14-15 1.0042706194226696 11102 16-17 0.4250641052307387 11102 18-19 0.21623002947423942 11102 20-21 1.015668353543326 11102 22-23 -0.776529635033377 11102 24-25 0.7066172044176184 11102 26-27 -0.29993452692124123 11102 28-29 0.5690915942631065 11102 30-31 0.2734516657470465 11102 32-33 1.0538388965804373 11102 34-35 1.14533208328357 11102 36-37 0.13227834202553623 11102 38-39 0.2793003309224176 11102 40-41 -0.5849986239150837 11102 42-43 0.651425735975188 11102 44-45 0.8402013362041743 11102 46-47 0.13570821027301605 11102 48-49 0.14645040722233205 11102 50-51 0.24793201963521483 11102 52-53 0.6502921438983762 11102 54-55 0.6178139995116609 11102 56-57 0.08533559221224607 11102 58-59 -0.4950025080927105 11102 60-61 0.9516138354378398 11102 62-63 -0.2943241121993694 11102 64-65 0.2881697379956023 11102 66-67 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66-67 0.9544234976531527 11105 68-69 -0.405221542348535 11105 70-71 0.09129198779157832 11105 72-73 0.08608575340961266 11105 74-75 0.3803118539105199 11105 76 0.2575049257912454 11106 1 -1.285329152703543 11106 2 -1.166888516191971 11106 3 0.11985353475780514 11106 4 -1.0053225885357904 11106 5 -0.6281758302093863 11106 6 -1.3012544696744541 11106 7 0.07120287318392826 11106 8 1.0673611546173731 11106 9 0.8504974864505996 11106 10-11 -0.2184572725377052 11106 12-13 -0.2437276693743584 11106 14-15 -0.8104519979315299 11106 16-17 -0.24314357613483395 11106 18-19 -1.170683199544257 11106 20-21 -1.4309888299559574 11106 22-23 -0.4472266478356559 11106 24-25 -0.32289915404611946 11106 26-27 -0.8347851670350366 11106 28-29 -0.648191478283124 11106 30-31 -0.4409864565858115 11106 32-33 -0.2580387705603897 11106 34-35 -0.3304858399027708 11106 36-37 -0.19097912596877364 11106 38-39 0.04352508198927296 11106 40-41 -0.1372745840857803 11106 42-43 -0.5185600392737442 11106 44-45 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1.2063262818087352 21612 7 1.890583229149648 21612 8 0.052858847432140976 21612 9 1.822152067861282 21612 10-11 1.2146343556758836 21612 12-13 1.2361668593006598 21612 14-15 0.3685196566498803 21612 16-17 0.5511873401473011 21612 18-19 1.0442146251096673 21612 20-21 0.2005207283828625 21612 22-23 1.2587720337727788 21612 24-25 1.762137101721855 21612 26-27 0.8553928158258728 21612 28-29 -0.09875179469709394 21612 30-31 0.3661987774286857 21612 32-33 1.0201418490836502 21612 34-35 0.043278168007581996 21612 36-37 0.3489022187906201 21612 38-39 1.198106492668245 21612 40-41 0.25165092679095125 21612 42-43 0.46528966408815364 21612 44-45 1.265425983187484 21612 46-47 0.28525891630638966 21612 48-49 -0.2187614027905056 21612 50-51 0.564363341843169 21612 52-53 0.6056836714978644 21612 54-55 0.5879680431573533 21612 56-57 0.7153099182713021 21612 58-59 0.5053184161691604 21612 60-61 1.2654777635508054 21612 62-63 0.9078581727813742 21612 64-65 1.2166036275976637 21612 66-67 0.6248648449237635 21612 68-69 0.7466367619487855 21612 70-71 0.9275289215894844 21612 72-73 1.5108825013770968 21612 74-75 0.7470365925864826 21612 76 0.9166222312500096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 7.0 13 31.0 14 55.0 15 67.0 16 151.0 17 207.0 18 273.0 19 306.0 20 352.0 21 325.0 22 384.0 23 414.0 24 474.0 25 527.0 26 553.0 27 661.0 28 724.0 29 876.0 30 1062.0 31 1302.0 32 1792.0 33 2459.0 34 4130.0 35 7862.0 36 35773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 84.26526246503208 3.4885634359058746 2.5670561132137566 9.679117985848281 2 80.85239427349022 3.880204048050025 10.779990126707256 4.487411551752509 3 80.61708079644562 4.204377159782788 10.61214415007405 4.566397893697548 4 80.61214415007404 7.465854862596675 6.31561625802205 5.606384729307224 5 80.22544018430146 6.279414184630575 10.613789698864572 2.8813559322033897 6 82.88107423192747 10.69788872615972 3.0064671131662526 3.414569928746565 7 82.07967878359031 5.774325725287564 3.640013822837302 8.505981668284816 8 82.20638812552453 10.291431486448682 3.498494298079613 4.003686089947177 9 80.95606384729307 3.7189402665789038 3.0656573967418135 12.259338489386211 10-11 82.53249958861281 6.59700510120125 6.343590587460918 4.526904722725029 12-13 81.54846141188085 4.225769294059569 6.86852065163732 7.357248642422248 14-15 84.50057594207668 5.812078328122429 4.052163896659536 5.635181833141353 16-17 84.41747572815534 4.742471614283363 3.7814711206187264 7.058581536942571 18-19 81.75318210615524 4.073589547388081 6.358452842297534 7.814775504159159 20-21 84.60196651170445 4.1996132801250665 4.306578351915086 6.8918418562554 22-23 84.69627033297954 4.026691020989148 3.501756637787048 7.775282008244268 24-25 82.79550428672515 6.439961164409485 3.7782421958564396 6.986292353008936 26-27 84.97437036671357 6.12971967845712 4.159158788537013 4.736751166292301 28-29 84.75715613918166 6.658767967483688 4.06865286039872 4.51542303293593 30-31 84.76101927807993 4.513028954145651 4.3163810197717565 6.409570748002666 32-33 82.83100063357277 4.073790657681453 8.389490920161602 4.70571778858417 34-35 84.31341895883558 6.276278005874754 5.292215539300789 4.118087495988876 36-37 85.19969063173659 4.416725633135315 6.504961411245866 3.878622323882243 38-39 85.00234492631995 3.8785904113083047 7.08743695439324 4.031627707978509 40-41 84.57766295315045 5.980022708946995 5.073310404976222 4.3690039329263275 42-43 82.45404729385048 6.328884793233391 6.790468824565156 4.426599088350969 44-45 81.94668421918709 3.8991278591410237 7.475728155339806 6.678459766332072 46-47 83.90050848294361 3.7190014645625236 5.466603037733055 6.913887014760816 48-49 84.85342153547421 3.7214392088136314 7.101424234196431 4.323715021515728 50-51 86.51861969096085 3.8753311721436914 5.330020240583192 4.276028896312265 52-53 82.1917244670435 4.077703453212549 6.759970050765599 6.970602028978353 54-55 83.9046224226168 3.758495285425135 5.144892955289704 7.1919893366683665 56-57 83.80862267566233 4.08672042126049 4.860128352805661 7.244528550271516 58-59 82.064193386485 6.439908177621997 6.62009725273369 4.875801183159315 60-61 81.39923316164491 4.643815103095329 7.340913952837795 6.616037782421959 62-63 81.50552497552226 6.520540731781568 6.791235735031553 5.182698557664618 64-65 81.68735961296375 6.824969762792191 6.987880433441118 4.499790190802952 66-67 81.6082212970429 9.512251312346756 4.215142589149073 4.664384801461272 68-69 82.00988982960202 10.045335242185637 3.604604283398744 4.3401706448135995 70-71 81.85902235533213 10.052905699498918 3.6951710178793293 4.392900927289632 72-73 82.70102600811262 9.50312245452077 3.5700474744732147 4.225804062893392 74-75 84.07839845967762 8.062830671504859 3.708457785128319 4.150313083689204 76 87.55615527653903 4.479257516167783 3.444190294393523 4.5203969128996695 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.5 16 5.5 17 9.0 18 11.5 19 15.5 20 13.5 21 10.0 22 12.5 23 31.0 24 55.0 25 63.0 26 58.0 27 60.5 28 84.0 29 100.0 30 93.5 31 105.0 32 108.0 33 93.0 34 136.5 35 186.5 36 210.5 37 228.0 38 256.0 39 333.0 40 367.0 41 348.5 42 345.0 43 487.0 44 1323.5 45 1751.5 46 1485.0 47 1001.0 48 459.5 49 397.0 50 392.0 51 344.0 52 373.0 53 464.0 54 478.0 55 455.5 56 440.5 57 421.5 58 395.0 59 383.5 60 337.0 61 314.0 62 326.0 63 309.5 64 292.5 65 227.5 66 167.5 67 172.0 68 130.5 69 104.5 70 96.5 71 73.0 72 78.0 73 72.5 74 53.5 75 45.0 76 48.5 77 45.0 78 43.5 79 49.0 80 41.5 81 37.0 82 36.0 83 32.0 84 36.0 85 46.0 86 51.0 87 50.0 88 65.0 89 78.5 90 88.0 91 111.0 92 123.0 93 140.0 94 172.0 95 229.5 96 272.0 97 354.0 98 692.0 99 22975.0 100 45002.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.001645548790521639 7 0.001645548790521639 8 0.001645548790521639 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.227743952608195E-4 20-21 0.004113871976304097 22-23 8.227743952608195E-4 24-25 0.001645548790521639 26-27 8.227743952608195E-4 28-29 8.227743952608195E-4 30-31 0.002468323185782458 32-33 0.005759420766825736 34-35 0.002468323185782458 36-37 0.001645548790521639 38-39 8.227743952608195E-4 40-41 0.001645548790521639 42-43 0.001645548790521639 44-45 0.0 46-47 0.001645548790521639 48-49 8.227743952608195E-4 50-51 0.001645548790521639 52-53 8.227743952608195E-4 54-55 0.001645548790521639 56-57 0.0 58-59 8.227743952608195E-4 60-61 0.001645548790521639 62-63 8.227743952608195E-4 64-65 8.227743952608195E-4 66-67 0.001645548790521639 68-69 8.227743952608195E-4 70-71 0.002468323185782458 72-73 8.227743952608195E-4 74-75 0.005759420766825736 76 0.001645548790521639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 60770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.69277604080961 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42320714065866 8.064834622346552 2 5.140043090181594 1.099226592068455 3 3.893505694059711 1.248971532005924 4 3.1855955678670362 1.362514398551917 5 2.923976608187134 1.563271350995557 6 2.7085257002154512 1.7376995227908507 7 1.7082179132040627 1.2785914102353135 8 1.6774392120652508 1.4349185453348692 9 1.2003693444136658 1.1551752509461906 >10 2.0006155740227762 2.9883166035872963 >50 0.06155740227762388 0.44594372223136414 >100 0.04616805170821791 0.9922659206845483 >500 0.0 0.0 >1k 0.01538935056940597 3.4918545334869178 >5k 0.0 0.0 >10k+ 0.01538935056940597 73.13641599473424 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 44445 73.13641599473424 No Hit CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG 2122 3.4918545334869178 No Hit TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT 214 0.35214744117163077 No Hit ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC 202 0.33240085568537103 No Hit CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG 187 0.30771762382754647 No Hit TCTTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG 82 0.1349350008227744 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 69 0.11354286654599308 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 61 0.10037847622181997 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009873292743129834 0.0 2 0.0 0.0 0.0 0.009873292743129834 0.0 3 0.0 0.0 0.0 0.01974658548625967 0.0 4 0.0 0.0 0.0 0.01974658548625967 0.0 5 0.0 0.0 0.0 0.04278426855356261 0.0 6 0.0 0.0 0.0 0.04278426855356261 0.0 7 0.0 0.0 0.0 0.04278426855356261 0.0 8 0.0 0.0 0.0 0.04278426855356261 0.0 9 0.0 0.0 0.0 0.05759420766825736 0.0 10 0.0 0.0 0.0 0.05759420766825736 0.0 11 0.0 0.0 0.0 0.05759420766825736 0.0 12 0.0 0.0 0.0 0.05759420766825736 0.0 13 0.0 0.0 0.0 0.06911304920190883 0.0 14 0.0 0.0 0.0 0.08392298831660358 0.0 15 0.0 0.0 0.0 0.11025176896494981 0.0 16 0.0 0.0 0.0 0.12341615928912292 0.0 17 0.0 0.0 0.0 0.13328945203225276 0.0 18 0.0 0.0 0.0 0.1398716471943393 0.0 19 0.0 0.0 0.0 0.159618232680599 0.0 20 0.0 0.0 0.0 0.18759256211946684 0.0 21 0.0 0.0 0.0 0.1941747572815534 0.0 22 0.0 0.0 0.0 0.23366792825407273 0.0 23 0.0 0.0 0.0 0.25176896494981077 0.0 24 0.0 0.0 0.0 0.28961658713180843 0.0 25 0.0 0.0 0.0 0.28961658713180843 0.0 26 0.0 0.0 0.0 0.28961658713180843 0.0 27 0.0 0.0 0.0 0.319236465361198 0.0 28 0.0 0.0 0.0 0.3356919532664143 0.0 29 0.0 0.0 0.0 0.3653118314958039 0.0 30 0.0 0.0 0.0 0.4130327464209314 0.0 31 0.0 0.0 0.0 0.44100707585979926 0.0 32 0.0 0.0 0.0 0.5167023202237946 0.0 33 0.0 0.0 0.0 0.5677143327299654 0.0 34 0.0 0.0 0.0 0.651637321046569 0.0 35 0.0 0.0 0.0 0.6944215896001317 0.0 36 0.0 0.0 0.0 0.834293236794471 0.0 37 0.0 0.0 0.0 1.0268224452855028 0.0 38 0.0 0.0 0.0 1.2917558005594867 0.0 39 0.0 0.0 0.0 1.5961823268059898 0.0 40 0.0 0.0 0.0 1.9828862925785748 0.0 41 0.0 0.0 0.0 2.3087049531018593 0.0 42 0.0 0.0 0.0 2.5226262958696726 0.0 43 0.0 0.0 0.0 2.734902089846964 0.0 44 0.0 0.0 0.0 2.970215566891558 0.0 45 0.0 0.0 0.0 3.2795787395096263 0.0 46 0.0 0.0 0.0 3.6531183149580384 0.0 47 0.0 0.0 0.0 3.9147605726509793 0.0 48 0.0 0.0 0.0 4.150074049695573 0.0 49 0.0 0.0 0.0 4.461082771104163 0.0 50 0.0 0.0 0.0 4.663485272338325 0.0 51 0.0 0.0 0.0 4.852723383248313 0.0 52 0.0 0.0 0.0 5.0222149086720425 0.0 53 0.0 0.0 0.0 5.242718446601942 0.0 54 0.0 0.0 0.0 5.3611979595195 0.0 55 0.0 0.0 0.0 5.603093631726181 0.0 56 0.0 0.0 0.0 5.830179364818167 0.0 57 0.0 0.0 0.0 6.025999670890242 0.0 58 0.0 0.0 0.0 6.167516866875103 0.0 59 0.0 0.0 0.0 6.414349185453348 0.0 60 0.0 0.0 0.0 6.511436564094125 0.0 61 0.0 0.0 0.0 6.567385222971861 0.0 62 0.0 0.0 0.0 6.723712358071417 0.0 63 0.0 0.0 0.0 6.8306730294553235 0.0 64 0.0 0.0 0.0 7.0330755306894845 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCATAG 15 0.0022147472 70.0 29 ATAGGTG 15 0.0022147472 70.0 32 TCATGCC 15 0.0022147472 70.0 18 AAGGCAT 15 0.0022147472 70.0 27 ATAGAGT 15 0.0022147472 70.0 42 CATAGGT 15 0.0022147472 70.0 31 AGGCATA 15 0.0022147472 70.0 28 GCATAGG 15 0.0022147472 70.0 30 TAGGTGG 15 0.0022147472 70.0 45 CAGCCGA 25 2.3729929E-4 55.999996 18 AAAAAGG 195 0.0 53.846153 70 TAAAAAA 375 0.0 53.2 64 ACGCTGT 20 0.0069205416 52.5 57 ATGATGC 20 0.0069205416 52.5 20 CTAGGTG 20 0.0069205416 52.5 44 GTGACCT 20 0.0069205416 52.5 4 TAGAGTT 20 0.0069205416 52.5 43 AGCCGAC 20 0.0069205416 52.5 19 GAAGGCA 20 0.0069205416 52.5 26 GTCGCCG 370 0.0 52.027027 51 >>END_MODULE