FastQCFastQC Report
Wed 25 May 2016
SRR1780061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60770
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2836446.67434589435577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35365.818660523284516No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG32515.349679117985849No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC21883.600460753661346No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT8871.459601777192694No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT7511.2358071416817509No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT7401.2177061049860127TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC6030.9922659206845483No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4250.6993582359716966No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT3200.5265756129669245No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT3130.515056771433273No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC2630.43277933190719103No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT2360.3883495145631068No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT1800.296198782293895No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC1450.23860457462563764No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG1390.22873128188250783No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC1340.2205035379298996No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT1260.20733914760572653No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC1090.17936481816685865No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG940.15468158630903409No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCG890.14645384235642586No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCAGT830.13658054961329602No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGAACATCTCGTATGCCGT730.12012506170807964No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG670.1102517689649498No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGA660.10860622017442817No Hit
CTTATACACATCTCCGAGCCCACGAGCCCATCGACCATCTCGTATGCCGT610.10037847622181997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACTC252.7998685E-670.0370735
ACCAACT207.847764E-570.0370734
GAATGAC252.7998685E-670.0370742
TCTCGAA207.847764E-570.0370738
TCGTAAG207.847764E-570.0370740
GCCGTAT207.847764E-570.0370746
CTCGTAA207.847764E-570.0370739
AATGACG252.7998685E-670.0370743
CTCGAAT301.0043368E-770.0370639
ACTCGTA301.0043368E-770.0370638
CAACTCG301.0043368E-770.0370636
AACTCGT301.0043368E-770.0370637
AAGACGT150.002210093470.0370644
CGAATGC150.002210093470.0370641
ACTGCTA150.002217331269.9794253
CTTCAGA150.002217331269.9794251
TAAACAA207.879884E-569.979425
ACGTCTA150.002217331269.9794247
ACTTGGT150.002217331269.979423
TCGAAAA150.002217331269.9794231
Produced by FastQC (version 0.11.5)