FastQCFastQC Report
Wed 25 May 2016
SRR1780060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327564
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4619314.101977018231551No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG23270.7103955257598515No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC3380.10318594228914044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG401.0631993E-861.2562819
GGCAATC200.006944807252.5053828
TTCGTCA200.006944807252.5053830
TGGCGAT200.006949001552.49736426
ACGAGTG200.006949001552.4973645
CGTAAGG305.874667E-446.6643263
TGGTCGC4150.042.1665649
TCGCCGT4150.041.3232352
GCCGTAT4150.041.3232354
GTTCGCT602.59015E-740.83751759
ATTCCAC350.001253822440.004117
GACGGCC350.001253822440.004131
CACGTAC350.00125476439.99799339
ATCTCGG4400.038.97531542
TACTCGC459.1819486E-538.89287620
ACTCGCA459.190212E-538.8869421
CGTATCA4350.038.61875556
TCGGTGG4350.038.61875545
GTCGCCG4450.038.5373951
TCTCGGT4450.038.5373943