FastQCFastQC Report
Wed 25 May 2016
SRR1780060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327564
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT302029.220182926084673No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC43361.3237107862890916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41491.2666227057918453No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG28990.8850178896337815No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT6620.20209791063731056No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT6530.1993503559609725No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT6430.19629751743170798RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC5800.17706463469734157No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT4220.12882978593496233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTT301.0230542E-769.9896247
GCAATCG207.925989E-569.989628
AGGGGGG37300.063.33807470
CGACTTC352.9692637E-760.00026348
CGTCTTA308.366558E-658.33358448
AAGGGGG44000.053.6138769
ATCGTAT407.475428E-752.49221439
GCCGTAT200.00695169852.49221446
CCGTCTT45850.050.9084847
GCCGACT552.2810127E-950.9015446
TATGCCG47200.050.861143
CGTCTTC46200.050.83355348
CTGCTTG46450.050.71066354
CGTATGC48050.050.68967441
TCGTATG48350.050.6646740
GCCGTCT46300.050.64043846
CTTGAAA47800.050.5964657
CTCGTAT48150.050.4390439
TCTTCTG46350.050.36699350
GTATGCC47850.050.31646742