FastQCFastQC Report
Wed 25 May 2016
SRR1780059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121475
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5362044.14076970570076No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG23011.8942169170611236No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC3450.2840090553611854No Hit
TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT2760.22720724428894834No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG2320.19098579954723194No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1750.14406256431364478No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.10454826095904507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAGT150.00222010370.0000151
TCCTGGT150.00222010370.0000129
GACCGTA150.00222010370.000011
GTTACGA150.00222010370.0000154
TACGACT150.00222010370.0000156
TGTTACG150.00222010370.0000153
CGACTTG150.00222010370.0000158
CACAAAT150.00222010370.000018
AATACGT150.00222010370.0000145
ACGACTT150.00222010370.0000157
TCTATAT150.00222010370.0000170
AACCGTA207.9001475E-570.07
TCGGTTT207.9001475E-570.023
ATCCTCG252.3825032E-455.99999631
CAACCGT252.3825032E-455.9999966
CTGGTTA252.3825032E-455.99999631
GTATCGG252.3825032E-455.99999611
ACCGTAT252.3825032E-455.9999968
ATCGGTT252.3825032E-455.99999622
TATCGGT252.3825032E-455.99999621
Produced by FastQC (version 0.11.5)