FastQCFastQC Report
Wed 25 May 2016
SRR1780059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121475
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3621329.811072237085824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40523.335665774850792No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC36623.0146120600946698No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG34312.824449475200659No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT10660.8775468203334019RNA PCR Primer, Index 36 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT10060.8281539411401523No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC8190.6742128009878576No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT7770.6396377855525828No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC3910.3218769294093435No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT2040.16793578925704877No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT1910.15723399876517802No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG1630.13418398847499485No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC1500.1234821979831241No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT1480.12183576867668244RNA PCR Primer, Index 36 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC1420.11689648075735748RNA PCR Primer, Index 36 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCAACAATCTCGTATGCCGT1360.1119571928380325No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT1350.11113397818481169No Hit
CAAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT1330.10948754887837003No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATATCGTATGCCGT1250.10290183165260341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT150.00221709970.0238932
CGTCTTA353.6779966E-969.9950648
TCGTTGT150.00222072569.99505634
CGACCAA150.00222072569.99505612
ACGTGCA150.00222072569.99505640
AGCGCAG150.00222072569.99505610
AACAGCG150.00222072569.9950567
AACACGT150.00222072569.99505637
AGGGGGG42900.064.7739870
CGAATGC600.064.1621441
TCTCGAA550.063.6318738
CTCGAAT550.063.6318739
AACTCGT501.4551915E-1162.99555637
TAAGCCG401.0571966E-861.24567843
AAGCCGT352.9551302E-759.99576644
TCTTTAT1700.059.7016681
CGACTTC650.059.22658548
CACAACT650.059.2265857
AAACTCG601.8189894E-1258.32921636
TCGAATG601.8189894E-1258.32921640
Produced by FastQC (version 0.11.5)