FastQCFastQC Report
Wed 25 May 2016
SRR1780058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319385
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4627114.487530723108474No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG17820.5579473049767523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTG200.006947155652.50058752
GGACCGT200.006947155652.5005876
CAGTATA305.877203E-446.659881
CGGAATA350.001254338440.00044670
CCGTGCG350.001254338440.00044610
CGAGAAT459.186371E-538.88932415
TCGTTCG400.002416693635.00039324
TTGGACC400.002416693635.00039313
CGTGCGA400.002416693635.00039311
TGTACGA400.002416693635.00039359
CAATACG400.002416693635.00039317
TGCGCGT400.002418551134.994915
GCCGTAT2800.032.50036254
TCGGTGG3150.032.2225845
TCGTTTT552.9884538E-431.81853754
AGGCGCG552.9884538E-431.81853718
TAGCAAG552.9884538E-431.8185376
CGGTGGT3200.031.71910546
TCGCCGT2900.031.37966252
TGGTCGC3250.031.2311249
Produced by FastQC (version 0.11.5)