FastQCFastQC Report
Wed 25 May 2016
SRR1780058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319385
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT292909.170750035223946Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32841.0282261220783695No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC30770.9634140613992518No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG27510.8613428933732017No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT9340.29243702741205757Illumina PCR Primer Index 1 (95% over 24bp)
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT8140.25486481832271396Illumina PCR Primer Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC6770.21196987961238004No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT4670.14621851370602879TruSeq Adapter, Index 1 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4650.14559231022120636Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT3730.1167869499193763Illumina PCR Primer Index 1 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC3340.10457598196533964Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC401.3460522E-1069.99467548
CTTTCGA150.002224100369.99467510
TGCCGTA150.002224100369.99467545
AGGGGGG38150.062.3805870
CACGAAC750.056.01328333
AACTCGT653.6379788E-1253.8420637
CGAACTC850.053.5253435
AAGGGGG44900.052.9247169
GCCGTCT46950.052.7755446
TATGCCG47150.052.7743543
CCGTCTT46700.052.68335347
CGTCTTC46900.052.68256448
ATGCCGT47500.052.5328544
ATGTGCG200.006940950652.5124529
ACGATAT608.367351E-1152.5124534
AGACCGA200.006940950652.5124531
AGACGTG200.00694955452.4960068
CGCTCGA200.00694955452.49600611
CGAATGC407.471099E-752.49600641
TGCCGTC47500.052.4591745
Produced by FastQC (version 0.11.5)