FastQCFastQC Report
Wed 25 May 2016
SRR1780057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362985
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5526915.226249018554485No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG25200.6942435637836275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT453.8380676E-1062.2222181
CGTGACA200.006948224752.522
ACATTCG200.006948224752.513
ACTCGTC200.006948224752.546
GGTCCCA352.0846277E-550.01
TCGCCGT4550.049.23076652
GCCGTAT4600.048.69565254
GTCGCCG4850.047.62886451
TGGTCGC4950.047.37373749
ATCTCGG5050.047.1287142
GATCTCG5050.047.1287141
CGGTGGT5200.047.11538346
TCGGTGG5150.046.89320845
CGTATCA4850.046.18556656
CTCGGTG5100.045.98039244
CGCCGTA5000.044.853
GTGGTCG5250.044.66666848
AGATCTC5350.044.4859840
TCTCGGT5400.044.07407443
GTAGATC5500.043.27272438
Produced by FastQC (version 0.11.5)