FastQCFastQC Report
Wed 25 May 2016
SRR1780057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362985
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3665610.09848891827486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44601.2287009105059439No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC43511.1986721214375249No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG34090.9391572654517405No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT10850.2989104232957285TruSeq Adapter, Index 25 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT9490.2614433103296279No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT6850.1887130322189622No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC6440.17741779963359366No Hit
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC3750.10331005413446837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAT150.00222379669.9990439
TGGCATA150.00222379669.9990412
CGAATGC401.3460522E-1069.9990441
CGACTTA150.00222379669.9990448
AGGGGGG42950.063.39842670
AATCTCG401.0644726E-861.24915736
ATCTAGT900.054.4436937
AAGGGGG50300.054.06486569
CGTATGA1000.052.4992841
GCGCCGT200.006948602452.4992833
ACGGACC200.006948602452.4992852
GGCATAT200.006948602452.4992813
CGTCTTC49850.052.44661748
CCGTCTT49650.052.44640747
GCCGTCT50050.052.30697346
CGTATGC52900.052.00306341
TATGCCG52400.051.9649343
TCGTATG53400.051.90939740
ATGCCGT52600.051.9004344
GTCTTCT49500.051.82756849