FastQCFastQC Report
Wed 25 May 2016
SRR1780056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103981
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4892747.05378867293063No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG21632.080187726603899No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC3060.29428453275117566No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG2370.22792625575826353No Hit
TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT2280.21927082832440542No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1110.10675027168425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG150.002219200670.04
CGGGGAA150.002219200670.041
CTTGCGT150.002219200670.057
TCTGTAG150.002219200670.03
GGACCTA150.002219200670.01
CTCTAAC150.002219200670.022
GACCTAA150.002219200670.02
GTAAAGC150.002219200670.03
TGTCACA200.006934350452.539
TAATAGG200.006934350452.57
CTGTAGA200.006934350452.51
GTCTGCT200.006934350452.570
ACGTTTG200.006934350452.524
CCCTAGT200.006934350452.51
GACCGGG200.006934350452.57
AAGACTC200.006934350452.533
GTAGGCT200.006934350452.56
CATTCAT200.006934350452.513
TGTGAGT200.006934350452.54
GCATTTG200.006934350452.541
Produced by FastQC (version 0.11.5)