FastQCFastQC Report
Wed 25 May 2016
SRR1780056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103981
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3234231.10375934064877No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC38483.700676085054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38383.6910589434608245No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG33313.203469864686818No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT8880.854002173474Illumina PCR Primer Index 6 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT8510.81841874957925No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT6000.5770284955905406No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC5700.5481770708110135No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC2870.2760119637241419No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT1980.1904194035448784No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1860.17887883363306756No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC1840.17695540531443243No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCAAAATCTCGTATGCCGT1730.1663765495619392No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1720.16541483540262164No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT1530.14714226637558783Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT1470.14137198141968244No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1450.1394485531010473No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1400.13463998230445948No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC1160.11155884248083783No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1120.10771198584356757No Hit
CAAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1040.10001827256902703No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG150.002219200670.025
AGATCCA207.894794E-570.028
GGGTCAG150.002219200670.039
CTTTTCG207.894794E-570.047
CCAGCGC150.002219200670.08
ATCCGAG150.002219200670.012
ACGTGTG207.894794E-570.044
TCCACGA150.002219200670.026
ACTCGTA301.0151416E-770.038
TATAGTC207.894794E-570.04
TTTTCGC207.894794E-570.048
TTGGGCG150.002219200670.016
AGGGGGG37700.064.9867370
AAACTCG401.0544682E-861.2536
CGAATGC401.0544682E-861.2541
ACGATAC352.9495095E-760.027
AACTCGT352.9495095E-760.037
GCCGTAT352.9495095E-760.046
CAAATAC650.059.2307661
CAGCATG308.326819E-658.33333254
Produced by FastQC (version 0.11.5)