FastQCFastQC Report
Wed 25 May 2016
SRR1780055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426659
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4493710.532298627240959No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG19860.46547711404189296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG200.006946157752.5055415
CGTTCAT200.006946157752.5055419
GGTCCGA200.00694937852.4993821
ATGCGTT200.00694937852.49938223
GCCGTAT3150.041.11062654
CTTATAG350.001254953839.9995335
CGTATCA3450.038.55027456
CGGTGGT3450.038.55027446
TCGGTGG3550.038.45025345
TCGCCGT3500.036.9995752
TGGTCGC3550.036.47844749
GTCGCCG3750.035.4662551
TCTCGGT3750.035.4662543
GTATCAT3750.035.4662557
CTTGTAC501.7088027E-434.9995883
CTCGGTG3700.034.99958844
TAAGCGT400.002417873434.9995884
CTTAGTC400.002417873434.9995883
CCGTATC3750.034.5329355
GATCTCG3750.034.5329341
Produced by FastQC (version 0.11.5)