FastQCFastQC Report
Wed 25 May 2016
SRR1780055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426659
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT269256.310660269676721No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36210.8486871248467747No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG29290.6864967104877666No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC27960.6553242753580728No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT5810.13617432188234632Illumina PCR Primer Index 7 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT5480.12843980790279824No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC4930.11554895127021812No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT4680.10968947098268172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG34850.058.55397470
GTAGCGA252.3900987E-455.9963449
CGTCTTA3250.052.76578548
CGTTGCA200.0069508552.49657456
GTTACCG200.006954072552.4904218
TGCCGTA2000.052.49041745
TCGTATG42750.051.9788240
GCCGTCT36550.051.7962946
CCGTCTT36150.051.6918147
CTCGTAT42650.051.608439
CGTATGC42650.051.52635241
TATGCCG43150.051.49697543
TCTCGTA43300.051.399438
ATGCCGT42800.051.34576844
ATCTCGT43350.051.3401137
TGCCGTC41000.051.03946345
CGTCTTC34750.050.96069748
CATCTCG43900.050.93603536
GTATGCC43800.050.89253642
CTTGAAA39350.050.60660657
Produced by FastQC (version 0.11.5)