FastQCFastQC Report
Wed 25 May 2016
SRR1780054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189487
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3828320.203496809807532No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG47082.4846031653886547No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG5580.29447930464886773No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT5420.2860354536195095No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC5140.27125871431813264No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT150.00222202970.0000115
CGTGCTA150.00222202970.0000145
GTCCGAC150.00222202970.000011
GAACGCT150.00222202970.0000130
CGATCAT150.00222202970.0000127
CCGTTGC150.00222202970.0000122
GTTTCGA200.006943123452.546
ACCGCCA200.006943123452.521
GGGAACG200.006943123452.528
GGAACGC200.006943123452.529
AGTCTAA200.006943123452.51
GCGATCA200.006943123452.526
CCACGCG200.006943123452.525
GCGTATG200.006943123452.514
ATCGCAT200.006943123452.58
GCCGTAT7500.049.93333454
CGCCGTA7400.049.66216353
CGTATCA7350.049.5238156
CCGTATC7400.049.1891955
GTATCAT7600.048.35526757
Produced by FastQC (version 0.11.5)