FastQCFastQC Report
Wed 25 May 2016
SRR1780054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189487
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2502613.207238491294918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79074.172845630570963No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG28511.504588705293767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC25851.3642096819306864No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT10410.5493780575976188No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT7150.3773345928744452TruSeq Adapter, Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC5770.3045063777462306No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC2800.14776739301376873No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2360.12454680268303366No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT1930.10185395304163347No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC150.002220201870.0145223
ACTTACG150.002220201870.0145221
TTACGAG150.002220201870.0145223
AGCGTTC150.00222252869.9960413
CAGCGTT150.00222252869.9960412
ATTGTCG150.00222252869.9960447
CGGTCGT150.00222252869.9960412
AGGGGGG28200.063.66660770
TCGCAGT200.00693742952.5108926
CGGTTCT200.00693742952.5108934
TAGCGGT200.00693742952.5108920
ATCGCAG200.00693742952.5108925
GCCGTCT35000.052.49703246
GAAGCGA200.006944676452.4970355
GGTCGTG200.006944676452.4970313
GCGTTCC200.006944676452.4970314
TCGTGTA200.006944676452.4970315
TACGCCA200.006944676452.4970354
GAACCGA200.006944676452.497036
AACTATG200.006944676452.4970314
Produced by FastQC (version 0.11.5)