FastQCFastQC Report
Wed 25 May 2016
SRR1780053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608220
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388026.37959948702772No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG43120.7089539969090132No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG6750.11097957975732466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG150.00222430670.0007418
TTATCCG200.006950180552.50055348
ACGTCCA200.006950180552.50055319
GTTGCGT200.006950180552.50055319
TGCGTTA200.00695244152.4962421
CGATACC305.876902E-446.6671645
TGGTCGC8200.040.5492149
GCCGTAT7900.040.31688354
CGGTGGT8200.040.12237546
CGTGCCG350.001255745239.997139
ATTCGCA350.001255745239.9971339
ATCTCGG8100.039.75350642
GATCTCG8200.039.6955441
TCTCGGT8300.039.6389743
TCGCCGT8050.039.56563652
TCGGTGG8250.039.45496445
GTCGCCG8500.038.7062951
CGTATCA8250.038.60964656
CTCGGTG8350.038.56328244
AGATCTC8450.038.5211240