FastQCFastQC Report
Wed 25 May 2016
SRR1780053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608220
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT250144.1126566045181026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76691.2608924402354411No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC39800.6543684850876328No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG24770.40725395416132315No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT9650.158659695504916No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT7120.11706290486994837TruSeq Adapter, Index 9 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG34000.059.28938370
GCTCGAT200.006952665752.4958082
TATGCCG40600.050.08220743
TCGTATG41300.050.08074640
CTCGTAT41100.049.8986939
CGTATGC41000.049.84967841
CGTCTTC40400.049.80628248
CCGTCTT40400.049.7196647
GCCGTCT40700.049.615246
AAGGGGG39950.049.58302369
CTTGAAA41450.049.56178357
CTGCTTG41000.049.5082454
TCTTCTG40950.049.39370350
ATGCCGT41100.049.3026344
TGCCGTC41050.049.27742845
ATCTCGT41850.049.08807437
GTATGCC41550.048.93712242
TCTCGTA42100.048.79657738
GTCTTCT41650.048.47953849
CGTATGA651.8553692E-1048.4576741
Produced by FastQC (version 0.11.5)