FastQCFastQC Report
Wed 25 May 2016
SRR1780052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472711
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440929.327474926540742No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG58161.2303500447419247No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG7810.16521722574680936No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT6350.1343315471821049No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6140.12988908656663375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA150.002223745270.0027533
AACGTGA200.00694843752.50206418
GGCGTTA200.00695134452.4965131
TAGTCGT305.877798E-446.66356329
TCGCCGT10250.045.07494452
TGGTCGC10450.044.88214549
GCCGTAT10400.044.42012454
TCGGTGG10550.044.12495445
GTATCAT10250.044.0505157
GTGGTCG10850.043.550148
CGGTGGT10800.043.4276346
CGTATCA10400.043.4151756
CGCCGTA10700.043.17926453
GTCGCCG10800.043.10354651
CCGTATC10500.043.0016955
TCTCGGT10800.042.13128743
CTCGGTG10850.041.9371344
GATCTCG10800.041.80719841
GGTGGTC11400.041.44899747
ATCTCGG11100.040.67727342
Produced by FastQC (version 0.11.5)