FastQCFastQC Report
Wed 25 May 2016
SRR1780052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472711
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT286456.059727825246292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102292.1639014112216555No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC36400.7700265066816723No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG30590.6471184296536362No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT13290.2811442932362479No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT7800.1650056800032155TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC5130.10852296646365327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA150.002222612570.0117422
CGATCGG150.002222612570.0117422
AGGGGGG37400.060.45189370
AAGCCGT509.822543E-1055.99162344
CGAATGC509.822543E-1055.99162341
CCGTCTT42850.053.00257547
TATGCCG44000.052.65121543
ACGCGAA200.00694200152.51436627
CGTCTTC43400.052.497748
GCCGTCA800.052.4921546
GCCGTCT43500.052.45192346
AAGGGGG43000.052.4163169
CGTATGC44500.052.37418441
GTATGCC44450.052.11818742
TCGTATG45000.052.10331340
ATGCCGT44500.051.90234444
CTCGTAT45200.051.71792639
TGCCGTC44400.051.62515645
CTTGAAA45050.051.4349357
GTCTTCT44400.051.3153249