FastQCFastQC Report
Wed 25 May 2016
SRR1780051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622040
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG489187.864124493601697No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG46660.7501125329560799No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6860.11028229695839496No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG6730.10819239920262362No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT200.006949691652.5016343
CGGTTCT200.006949691652.5016343
GACCTTC200.006949691652.5016343
CGCGCAA200.006951901652.4974115
GCCGTAT8450.045.1493354
CGGTGGT8650.044.9110846
TCGGTGG8700.044.6529745
CTCGGTG8550.044.61768344
GTCGCCG8950.044.18776351
TCGCCGT8700.043.8484152
TGGTCGC8950.043.79672249
ATCTCGG8800.043.35361542
GATCTCG9000.042.39020541
TCTCGGT9400.040.95871743
GGTATCA602.5982627E-740.8313181
AGATCTC9500.040.1591440
GTAGATC9700.040.05278838
CGCCGTA9450.040.0012453
CGTATCA9550.039.94888356
GTATCAT9750.038.77043557