FastQCFastQC Report
Wed 25 May 2016
SRR1780051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622040
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT315785.076522410134396Illumina PCR Primer Index 6 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83731.346054916082567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC40860.6568709407755128No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG29170.4689409041219214No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT13020.20931129830879044Illumina PCR Primer Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT9440.1517587293421645Illumina PCR Primer Index 6 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG150.00222325870.0092523
TGCCGTA352.9743387E-759.9982845
AGGGGGG41800.059.28059470
CGAATGC308.377014E-658.33165741
GCCGTAT252.3905207E-455.9983946
CGCGATC200.006946913452.5069422
CGACTTT200.006951332652.49849361
CGTCTTA200.00695354352.49427448
AAGGGGG47150.052.25724869
CCGTCTT47500.051.6511547
GCCGTCT47800.051.40019646
TATGCCG49000.051.2842443
CGTCTTC48050.051.27432348
CGTATGC49850.050.8310441
TCTTCTG48150.050.8044350
ATGCCGT49600.050.7344344
TCGTATG50400.050.69298640
CTCGTAT50400.050.62354339
GTCTTCT48350.050.59427649
TCTCGTA50600.050.4926238