FastQCFastQC Report
Wed 25 May 2016
SRR1780050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792492
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501806.3319251172251585No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG62950.7943297850325304No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG10970.1384241102749302No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG200.00695094752.5005740
TGGTCGC12150.046.08812749
TCGCCGT12100.044.25388752
GCCGTAT12050.044.14985754
CGGTGGT12650.043.71312746
TCGGTGG12700.043.26545745
GTCGCCG13050.042.90963751
CGTATCA12400.042.9036956
TCTCGGT12750.042.82399443
CTCGGTG12700.042.71430244
CGCCGTA12500.042.5604653
GTGGTCG13400.042.0500448
CAAGCGT350.001255466140.0004314
GATCTCG13600.039.89013741
ATCTCGG13650.039.74402242
GTATCAT13500.039.40783357
AGATCTC13950.039.39110640
GGTCGCC14150.039.3265650
GTAGATC13900.039.28100238
GGTGGTC14750.038.4386747
Produced by FastQC (version 0.11.5)