FastQCFastQC Report
Wed 25 May 2016
SRR1780050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792492
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT324024.0886217147933355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114171.4406454576197614No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC49830.6287760633545828No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG31070.39205443083337116No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT16060.20265188796858516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG41900.060.39570270
GCCGTCT49800.051.37388246
TGCCGTC51000.051.1944645
ATGCCGT51350.050.9818444
CGTATGC51900.050.9810541
TATGCCG51750.050.9259343
AAGGGGG49300.050.8332569
CGTCTTC50000.050.81839848
TCGTATG52250.050.7735240
CCGTCTT50150.050.73618347
CTCGTAT52250.050.43860239
TCTCGTA52400.050.22742538
GTATGCC53000.049.9229542
ATCTCGT52650.049.8559837
GTCTTCT50650.048.99154749
CATCTCG53950.048.84924736
CTGCTTG50950.048.56569354
CTTGAAA52900.048.56179457
CTTCTGC52400.047.4221751
TCTTCTG52500.047.39850650
Produced by FastQC (version 0.11.5)