FastQCFastQC Report
Wed 25 May 2016
SRR1780049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119187
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4829940.523714834671566No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG57414.816800489986324No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT6750.5663369327191724No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6450.541166402376098No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG6020.5050886422176915No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG1660.13927693456501128No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG1280.10739426279711714No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATTG150.002220000170.051
AGTGATT150.002220000170.017
TGTAGTA150.002220000170.04
CCGAACG150.002220000170.035
AGGGAGT150.002220000170.033
AAAAAGG4850.056.28866670
CCCTATC252.382319E-455.99999625
ATGAGAG252.382319E-455.9999965
CTAGACT200.006936829552.54
CTCACAA200.006936829552.520
ACACGAC200.006936829552.528
GACAAGC200.006936829552.557
GAGAGTA200.006936829552.57
ATAGAAT200.006936829552.529
CTTATAA200.006936829552.51
GTATGCC200.006936829552.58
CAAGAGC200.006936829552.532
TGCTACT200.006936829552.543
GGATTTG200.006936829552.51
CGTCCGG200.006936829552.510