##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780049_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 119187 Sequences flagged as poor quality 0 Sequence length 76 %GC 70 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.690016528648258 32.0 27.0 32.0 27.0 32.0 2 30.342797452742328 32.0 32.0 32.0 27.0 32.0 3 30.230931225720926 32.0 32.0 32.0 27.0 32.0 4 29.65947628516533 32.0 32.0 32.0 27.0 32.0 5 30.225351758161544 32.0 32.0 32.0 27.0 32.0 6 34.46585617558962 37.0 37.0 37.0 27.0 37.0 7 34.31331437153381 37.0 37.0 37.0 27.0 37.0 8 34.347839948987726 37.0 37.0 37.0 27.0 37.0 9 34.640187268745755 37.0 37.0 37.0 27.0 37.0 10-11 34.38739543742187 37.0 37.0 37.0 27.0 37.0 12-13 34.2217020312618 37.0 37.0 37.0 27.0 37.0 14-15 34.58175388255431 37.0 37.0 37.0 29.5 37.0 16-17 34.79830015018416 37.0 37.0 37.0 29.5 37.0 18-19 34.483924421287554 37.0 37.0 37.0 27.0 37.0 20-21 34.739107452994034 37.0 37.0 37.0 29.5 37.0 22-23 34.78396972824218 37.0 37.0 37.0 29.5 37.0 24-25 34.26328794247695 37.0 37.0 37.0 27.0 37.0 26-27 34.62072205861377 37.0 37.0 37.0 29.5 37.0 28-29 34.578544639935565 37.0 37.0 37.0 29.5 37.0 30-31 34.546418653041016 37.0 37.0 37.0 27.0 37.0 32-33 34.595761282690226 37.0 37.0 37.0 32.0 37.0 34-35 34.462902833362705 37.0 37.0 37.0 27.0 37.0 36-37 34.94377323030196 37.0 37.0 37.0 32.0 37.0 38-39 35.02279191522565 37.0 37.0 37.0 32.0 37.0 40-41 34.552426019616234 37.0 37.0 37.0 29.5 37.0 42-43 34.53451718727714 37.0 37.0 37.0 27.0 37.0 44-45 34.3345834696737 37.0 37.0 37.0 27.0 37.0 46-47 34.49682431808838 37.0 37.0 37.0 29.5 37.0 48-49 34.85627207665266 37.0 37.0 37.0 32.0 37.0 50-51 35.058353679512024 37.0 37.0 37.0 32.0 37.0 52-53 34.44942820945237 37.0 37.0 37.0 27.0 37.0 54-55 34.566831953149254 37.0 37.0 37.0 29.5 37.0 56-57 34.40397023165278 37.0 37.0 37.0 27.0 37.0 58-59 34.07823839848305 37.0 37.0 37.0 24.5 37.0 60-61 33.98176395076644 37.0 37.0 37.0 24.5 37.0 62-63 33.5891833840939 37.0 37.0 37.0 22.0 37.0 64-65 33.38953073741264 37.0 37.0 37.0 17.5 37.0 66-67 33.46466477048671 37.0 37.0 37.0 22.0 37.0 68-69 33.6866814333778 37.0 37.0 37.0 22.0 37.0 70-71 33.746222322904345 37.0 37.0 37.0 22.0 37.0 72-73 33.8662899477292 37.0 37.0 37.0 22.0 37.0 74-75 33.551071006066095 37.0 37.0 37.0 17.5 37.0 76 33.281372968528444 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.22189045497549742 11101 2 -0.04236304462017415 11101 3 -0.5290637357640264 11101 4 -0.9009396674313805 11101 5 -0.649781834553611 11101 6 0.5075600054026523 11101 7 -0.570573105948263 11101 8 0.007288094185355476 11101 9 0.02954785673516369 11101 10-11 -0.028995405080650016 11101 12-13 -0.21321647514317021 11101 14-15 0.3493275787987571 11101 16-17 0.42433653144064465 11101 18-19 0.46681207734481234 11101 20-21 0.056635036097191005 11101 22-23 -0.27746186067666656 11101 24-25 -1.7827121204415732 11101 26-27 0.4614907528157133 11101 28-29 0.45020668223710203 11101 30-31 0.015686058394052793 11101 32-33 0.3337136268834229 11101 34-35 0.827202832478136 11101 36-37 -0.4729174571818575 11101 38-39 -0.11666484139122701 11101 40-41 0.024942538637127143 11101 42-43 0.490825846979277 11101 44-45 0.39685502057790956 11101 46-47 -0.0026544915816586467 11101 48-49 0.6673631372078432 11101 50-51 -0.3490181845114506 11101 52-53 0.20745419981458468 11101 54-55 -0.2599420230829139 11101 56-57 -1.4272296821883685 11101 58-59 -0.19723926472220654 11101 60-61 0.04241988263242291 11101 62-63 -0.265353752594919 11101 64-65 -0.4069883756170469 11101 66-67 0.6757676190789113 11101 68-69 -0.3329609040900934 11101 70-71 -0.33717201690262044 11101 72-73 -0.44039759694958036 11101 74-75 -0.3200373671169672 11101 76 0.43613576318101366 11102 1 0.23939045497549571 11102 2 0.05347028871316084 11102 3 -0.159897069097358 11102 4 0.12822699923528447 11102 5 -0.013115167886944334 11102 6 0.08339333873598775 11102 7 0.12109356071839983 11102 8 0.12312142751869004 11102 9 -0.26128547659817514 11102 10-11 0.6497545949193437 11102 12-13 0.5147001915235023 11102 14-15 -0.4915057545345789 11102 16-17 -0.015663468559353078 11102 18-19 0.6405620773448106 11102 20-21 0.4603850360971933 11102 22-23 -0.11412852734333256 11102 24-25 0.14770454622509277 11102 26-27 0.21565741948237616 11102 28-29 0.27937334890377485 11102 30-31 0.4636027250607242 11102 32-33 -0.3554530397832423 11102 34-35 -0.0519638341885269 11102 36-37 -0.027917457181864336 11102 38-39 0.3441684919421135 11102 40-41 0.15744253863712032 11102 42-43 0.2658258469792827 11102 44-45 0.09893835391124384 11102 46-47 0.9619288417516714 11102 48-49 0.621529803874509 11102 50-51 0.4151484821552174 11102 52-53 -0.3421291335187462 11102 54-55 -0.45619202308291307 11102 56-57 0.6694369844782955 11102 58-59 0.10984406861112461 11102 60-61 -0.5392467840342476 11102 62-63 -0.032437085928258114 11102 64-65 -0.6182383756170537 11102 66-67 -0.15881571425443042 11102 68-69 0.42953909590990946 11102 70-71 -1.4055053502359485 11102 72-73 -0.6508142636162475 11102 74-75 -1.064204033783632 11102 76 -1.1663642368189855 11103 1 -0.9822004541154143 11103 2 -0.22948425674138662 11103 3 -0.31330616000644795 11103 4 -0.22518209167380476 11103 5 0.5323393775676024 11103 6 0.475438793281441 11103 7 0.8551844698093092 11103 8 0.7742577911550583 11103 9 0.3193963415836478 11103 10-11 0.7446409585557063 11103 12-13 0.46413200970531676 11103 14-15 -0.48127848180730837 11103 16-17 -0.5338452867411689 11103 18-19 -0.25432428629154913 11103 20-21 0.20299867246083636 11103 22-23 -0.6334467091615181 11103 24-25 0.17327272804327265 11103 26-27 0.047475601300568826 11103 28-29 -0.05130846927804811 11103 30-31 -0.18242000221200527 11103 32-33 -0.2974984943286927 11103 34-35 0.8332634385387365 11103 36-37 0.13230981554541188 11103 38-39 -0.32742241714879583 11103 40-41 -0.20505746136287684 11103 42-43 0.115825846979277 11103 44-45 0.2415519902748855 11103 46-47 -0.5943211582483272 11103 48-49 -0.44778837794368087 11103 50-51 0.14810302760976413 11103 52-53 -0.3404245880642023 11103 54-55 0.07960343146253734 11103 56-57 -0.06806301552170169 11103 58-59 0.046775886792936205 11103 60-61 0.8084804886930286 11103 62-63 0.3419947322535677 11103 64-65 0.4652843516556757 11103 66-67 -0.02302025970897148 11103 68-69 0.23067545954626922 11103 70-71 -0.18505080478140457 11103 72-73 -0.32410971816170075 11103 74-75 -0.21988585196544363 11103 76 0.6484084904537397 11104 1 0.01953751379902613 11104 2 -0.06197088775742898 11104 3 0.4834852838438195 11104 4 0.3539622933529323 11104 5 -0.016056344357533447 11104 6 0.05030510344187178 11104 7 1.0960935607184012 11104 8 -0.31584916071660274 11104 9 0.8115086410488885 11104 10-11 -1.0454659933159434 11104 12-13 0.8158031326999691 11104 14-15 0.9901118925242471 11104 16-17 0.5549247667347643 11104 18-19 -0.45318792265518937 11104 20-21 0.42251738903837577 11104 22-23 -0.028834409696273156 11104 24-25 0.5216016050486232 11104 26-27 0.22227506654120788 11104 28-29 0.5639321724331836 11104 30-31 0.6411762544724837 11104 32-33 0.23057637198147063 11104 34-35 0.23112440110558907 11104 36-37 0.3764943075240197 11104 38-39 0.3684331978244586 11104 40-41 -0.23741040253934642 11104 42-43 -0.0797623883148475 11104 44-45 0.5890118833230105 11104 46-47 0.977737665281083 11104 48-49 0.3693239215215698 11104 50-51 0.0776484821552188 11104 52-53 0.908238513540077 11104 54-55 0.8294697416229653 11104 56-57 -0.13350419199228725 11104 58-59 -0.2662588725653521 11104 60-61 0.441635568906932 11104 62-63 -0.21515767416354237 11104 64-65 0.9306586832064738 11104 66-67 -0.5128598319014799 11104 68-69 -0.0035491393842121965 11104 70-71 -0.7657994678830065 11104 72-73 1.0399945599131613 11104 74-75 -0.25023344554833926 11104 76 1.0431945867104275 11105 1 -0.6083659552809131 11105 2 -0.10390150615863547 11105 3 -0.33778168448197476 11105 4 -0.04452941102112362 11105 5 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11105 64-65 -0.0023729910016641043 11105 66-67 -0.9326939193826291 11105 68-69 -0.5880891092182949 11105 70-71 0.08359721386661789 11105 72-73 -0.2750129815649629 11105 74-75 -0.16131941839901742 11105 76 -1.9346334675882169 11106 1 -0.031442878357836435 11106 2 -0.2962091984663253 11106 3 0.5852952385949486 11106 4 -0.12145248794420027 11106 5 0.12150021672843891 11106 6 0.41832923617188555 11106 7 -0.20134233671749513 11106 8 -0.40348113658387064 11106 9 -0.7519906048033036 11106 10-11 0.5220302359449818 11106 12-13 0.512937371010679 11106 14-15 -0.7604160109448372 11106 16-17 -0.9015609044567867 11106 18-19 -0.786521255988518 11106 20-21 -0.3372111177489572 11106 22-23 0.10766634445154466 11106 24-25 0.12446736673791037 11106 26-27 -0.052611811286844556 11106 28-29 -0.5151779331475126 11106 30-31 0.11055785326585266 11106 32-33 -0.41346586029605703 11106 34-35 0.7528438581191637 11106 36-37 -0.0747123289767373 11106 38-39 -0.7484597131860937 11106 40-41 -0.5127497690551834 11106 42-43 -0.1630203068668763 11106 44-45 0.01864989237278536 11106 46-47 -0.5270134659406338 11106 48-49 -0.18904711920241368 11106 50-51 -1.4133771588704178 11106 52-53 -0.4692124668520776 11106 54-55 -0.788403561544456 11106 56-57 0.1771292921706049 11106 58-59 -0.5390341365170812 11106 60-61 0.010112190324733206 11106 62-63 -0.44894349618466123 11106 64-65 -0.8421165807452553 11106 66-67 0.15063941395069946 11106 68-69 -0.00475577588496634 11106 70-71 0.11564849591789539 11106 72-73 -0.1852693918213788 11106 74-75 0.3835523764727782 11106 76 0.5012639683092175 11107 1 -1.19193670551833 11107 2 -0.33372106931152956 11107 3 -0.07424892094920921 11107 4 -0.2021742353326168 11107 5 0.144292239520464 11107 6 0.17521432639031076 11107 7 -0.8347706368124648 11107 8 -0.043082276185010926 11107 9 0.26510341229071344 11107 10-11 0.3038441010921815 11107 12-13 -0.6850683269950224 11107 14-15 -1.1274625446580373 11107 16-17 -0.56134248090504 11107 18-19 -0.4717064411737084 11107 20-21 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11108 4 0.20523317207479153 11108 5 -0.23842380986225464 11108 6 0.7678069189828989 11108 7 0.5849824496072884 11108 8 0.37667081023474225 11108 9 -0.03119288400557707 11108 10-11 0.5754490393637894 11108 12-13 -0.08630289489625653 11108 14-15 0.014512763983937305 11108 16-17 0.4016204820579219 11108 18-19 0.3245898551225892 11108 20-21 1.0758942953564556 11108 22-23 0.6864887566072895 11108 24-25 0.8039545462250928 11108 26-27 0.26149075281571754 11108 28-29 0.5662560649531514 11108 30-31 -0.3077707317294056 11108 32-33 0.6045778244142923 11108 34-35 0.7333756719843052 11108 36-37 0.040909703311960754 11108 38-39 0.09247096107791464 11108 40-41 0.643707970735889 11108 42-43 0.780949303769404 11108 44-45 0.6129044032939603 11108 46-47 0.3887035331096982 11108 48-49 0.5550174581954934 11108 50-51 -0.3680305301904667 11108 52-53 0.2314048170985359 11108 54-55 0.27783575469485555 11108 56-57 0.47128883633014595 11108 58-59 0.3111557970061867 11108 60-61 0.7147655616447679 11108 62-63 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-0.7568211582483286 21611 48-49 -0.5409701961254996 21611 50-51 0.1401484821552188 21611 52-53 -1.385879133518749 21611 54-55 -0.4749420230829102 21611 56-57 0.04443698447829547 21611 58-59 -0.9026559313888782 21611 60-61 -0.9704967840342462 21611 62-63 -0.6949370859282524 21611 64-65 -1.5932383756170516 21611 66-67 -0.7088157142544276 21611 68-69 -1.076710904090092 21611 70-71 -1.2305053502359513 21611 72-73 -0.4258142636162532 21611 74-75 -0.6642040337836335 21611 76 0.8961357631810145 21612 1 0.10958276266780231 21612 2 -0.8346707369278654 21612 3 -0.20957655627684346 21612 4 -0.7881191546108681 21612 5 1.0573976526258768 21612 6 -1.4150040971614501 21612 7 0.38840125302609607 21612 8 -1.8265580596607975 21612 9 -1.2519906048033036 21612 10-11 -1.1702774563627045 21612 12-13 -0.685780577707277 21612 14-15 -0.7604160109448372 21612 16-17 -0.5297660326619251 21612 18-19 -0.49164946111672236 21612 20-21 -1.0551598356976797 21612 22-23 -1.0013080145228201 21612 24-25 -0.47809673582619183 21612 26-27 -0.45645796513300496 21612 28-29 -0.4767163946859654 21612 30-31 -2.2548267621187676 21612 32-33 -0.9903889372191372 21612 34-35 -0.44587409059879235 21612 36-37 0.008621004356598405 21612 38-39 -0.4792289439553201 21612 40-41 -0.16659592290133673 21612 42-43 -0.1181485119950807 21612 44-45 -0.23776036403747014 21612 46-47 0.12042243149526399 21612 48-49 0.08659390643860831 21612 50-51 0.4071356616423998 21612 52-53 -0.661520159159771 21612 54-55 -0.2627625359034269 21612 56-57 0.3373857024270137 21612 58-59 -0.04544439292734381 21612 60-61 -0.3680929378803981 21612 62-63 -0.5130460602872304 21612 64-65 -0.7267319653606421 21612 66-67 -0.5032067398954467 21612 68-69 -0.7675762887054773 21612 70-71 -0.1856335553641557 21612 72-73 -0.5506540072059849 21612 74-75 -0.6228578799374773 21612 76 1.1422896093348598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 21.0 13 38.0 14 63.0 15 126.0 16 203.0 17 303.0 18 404.0 19 473.0 20 498.0 21 611.0 22 757.0 23 962.0 24 1189.0 25 1353.0 26 1575.0 27 1879.0 28 2087.0 29 2338.0 30 2893.0 31 3570.0 32 4769.0 33 7373.0 34 12882.0 35 24434.0 36 48382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 63.92307885927156 10.10177284435383 6.134058244607214 19.84109005176739 2 53.263359258979584 11.42238666968713 22.744930235680066 12.569323835653218 3 53.031790379823306 11.938382541720154 21.648334130400126 13.381492948056414 4 50.31337836137097 14.728363468557285 18.68188110919998 16.276377060871756 5 50.024751021504024 18.05314337973101 23.390973847818973 8.531131750945994 6 58.17258883248731 23.76725259051055 9.754583210974536 8.305575366027604 7 56.161326319145545 16.67044224621813 10.943307575490616 16.22492385914571 8 55.19981206004011 23.78950724491765 10.632032016914597 10.378648678127648 9 54.90783390806045 10.079958384723167 9.828253081292424 25.183954625923967 10-11 56.26536451123025 16.207723996744612 15.509241779724297 12.017669712300838 12-13 55.629389111228576 12.544572814149193 15.914487318247794 15.911550756374437 14-15 58.42080092627552 14.52717158750535 11.850285685519394 15.201741800699741 16-17 58.539347996627136 13.923137268063076 11.779438107503786 15.758076627806 18-19 55.30020808162169 13.40154718754195 15.188280306081353 16.109964424755 20-21 58.264112632568136 13.199338837427844 12.875469861726408 15.66107866827762 22-23 58.29603184924467 13.156271893209382 11.732872431021969 16.814823826523977 24-25 56.005000671231045 16.704423412538596 11.697878909920794 15.592697006309573 26-27 58.23324341671946 16.592342125024647 12.158879720097831 13.015534738158067 28-29 55.026408413775165 16.546475871645463 16.212542633122318 12.214573081457056 30-31 54.749568945626315 13.742978323523614 15.902655127134821 15.604797603715248 32-33 54.809330033141755 13.114066367412006 13.749632923606159 18.32697067584008 34-35 55.27331459495743 12.84180056215128 18.74019381633595 13.144691026555355 36-37 57.88228384444351 12.975206611570247 17.319713051138987 11.822796492847253 38-39 58.34063993220595 13.083077077975744 16.786159274066677 11.790123715751628 40-41 57.963116782870614 15.644743134737848 14.178559742253768 12.213580340137767 42-43 55.2199922809726 15.536640209420568 16.679391874884633 12.5639756347222 44-45 54.28406020841374 12.666336650277717 17.302787240112096 15.746815901196449 46-47 56.726098082812435 12.460460628434786 15.30058312707136 15.51285816168142 48-49 57.82701899963503 12.407445474109903 17.439475108338613 12.326060417916459 50-51 60.06275852232207 12.537860672724374 15.174054217322357 12.225326587631201 52-53 54.41666317070101 12.922347610857072 17.061291269874566 15.599697948567353 54-55 56.52084539421246 12.802990259004757 14.154647738427848 16.52151660835494 56-57 56.24798110524263 13.32617368577817 13.673947972295519 16.751897236683686 58-59 53.86161126306781 16.11095262866444 16.61772355813602 13.409712550131728 60-61 53.07270538190147 13.81616206533458 17.49568952859594 15.615443024168005 62-63 53.34709339642826 16.64506168955116 16.33420172755914 13.67364318646144 64-65 53.82827609063183 16.599754164726413 16.689949700257163 12.882020044384593 66-67 53.51741612863981 20.197087732987654 12.992771771733977 13.29272436663856 68-69 53.750409021118074 20.81560908488342 12.123805448580802 13.310176445417705 70-71 53.84796096873388 20.488478141400243 12.5286840372022 13.134876852663682 72-73 54.25724713680413 20.01887821454042 12.458782564920082 13.26509208373537 74-75 56.001275349672355 18.372167171492578 12.362501363449486 13.264056115385584 76 60.68045475521249 12.697067584008053 12.85312749087553 13.769350169903932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 2.0 8 2.0 9 3.5 10 6.5 11 7.0 12 6.0 13 11.5 14 18.0 15 29.5 16 40.5 17 41.0 18 45.5 19 62.5 20 93.5 21 112.0 22 111.5 23 150.0 24 216.0 25 243.0 26 321.0 27 442.5 28 541.5 29 597.0 30 664.5 31 791.0 32 957.0 33 1064.0 34 1186.0 35 1430.5 36 1524.0 37 1495.0 38 1714.0 39 2068.5 40 2344.5 41 2919.5 42 3354.0 43 5354.5 44 6683.5 45 4700.5 46 3389.0 47 3244.5 48 2948.5 49 2802.0 50 2807.0 51 2768.5 52 2436.5 53 2182.5 54 2222.0 55 2174.0 56 2074.5 57 1795.0 58 1567.0 59 1550.5 60 1426.5 61 1234.0 62 1149.0 63 1010.5 64 818.5 65 689.5 66 611.5 67 609.0 68 596.5 69 524.0 70 433.0 71 402.0 72 381.0 73 324.0 74 266.5 75 245.0 76 201.0 77 152.0 78 153.5 79 160.0 80 156.5 81 139.5 82 122.5 83 119.0 84 99.5 85 78.0 86 87.0 87 98.0 88 100.5 89 97.0 90 97.0 91 118.5 92 134.0 93 146.5 94 172.0 95 235.0 96 285.0 97 353.5 98 684.0 99 24865.5 100 48785.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0016780353562049553 5 0.0 6 0.0016780353562049553 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.1950883905123883E-4 18-19 0.0025170530343074328 20-21 0.0025170530343074328 22-23 4.1950883905123883E-4 24-25 0.0025170530343074328 26-27 0.0020975441952561943 28-29 0.0012585265171537164 30-31 0.0020975441952561943 32-33 0.0016780353562049553 34-35 0.0016780353562049553 36-37 0.0016780353562049553 38-39 0.0020975441952561943 40-41 0.0 42-43 8.390176781024777E-4 44-45 8.390176781024777E-4 46-47 0.0016780353562049553 48-49 4.1950883905123883E-4 50-51 0.0 52-53 0.0016780353562049553 54-55 0.0 56-57 4.1950883905123883E-4 58-59 8.390176781024777E-4 60-61 4.1950883905123883E-4 62-63 0.0012585265171537164 64-65 0.0012585265171537164 66-67 0.0012585265171537164 68-69 0.0 70-71 4.1950883905123883E-4 72-73 0.0016780353562049553 74-75 0.0033560707124099106 76 0.0016780353562049553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 119187.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.204711923280225 #Duplication Level Percentage of deduplicated Percentage of total 1 68.22865820587917 15.832263585793752 2 7.343529666992081 3.4080898084522646 3 5.062009617818274 3.5238742480304057 4 4.458184184835665 4.13803518840142 5 3.735039953718769 4.333526307399297 6 3.051668655313302 4.248785521910946 7 2.5201576454423833 4.093567251461988 8 1.9127164913041905 3.550722813729686 9 1.4065155295223633 2.9374008910367744 >10 2.223668510684456 6.258232860966381 >50 0.03254149040026033 0.4757230234841048 >100 0.007231442311168963 0.24667119736212842 >500 0.010847163466753445 1.6125919773129618 >1k 0.0 0.0 >5k 0.0036157211555844816 4.816800489986324 >10k+ 0.0036157211555844816 40.523714834671566 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 48299 40.523714834671566 No Hit CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG 5741 4.816800489986324 No Hit TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT 675 0.5663369327191724 No Hit ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC 645 0.541166402376098 No Hit CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG 602 0.5050886422176915 No Hit TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG 166 0.13927693456501128 No Hit CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG 128 0.10739426279711714 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009229194459127253 0.0 2 0.0 0.0 0.0 0.009229194459127253 0.0 3 0.0 0.0 0.0 0.009229194459127253 0.0 4 0.0 0.0 0.0 0.009229194459127253 0.0 5 0.0 0.0 0.0 0.026009548021176807 0.0 6 0.0 0.0 0.0 0.026009548021176807 0.0 7 0.0 0.0 0.0 0.026009548021176807 0.0 8 0.0 0.0 0.0 0.026009548021176807 0.0 9 0.0 0.0 0.0 0.03439972480220158 0.0 10 0.0 0.0 0.0 0.041111866227021406 0.0 11 0.0 0.0 0.0 0.041111866227021406 0.0 12 0.0 0.0 0.0 0.04278990158322636 0.0 13 0.0 0.0 0.0 0.043628919261328836 0.0 14 0.0 0.0 0.0 0.05034106068614866 0.0 15 0.0 0.0 0.0 0.056214184432866 0.0 16 0.0 0.0 0.0 0.056214184432866 0.0 17 0.0 0.0 0.0 0.056214184432866 0.0 18 0.0 0.0 0.0 0.06208730817958334 0.0 19 0.0 0.0 0.0 0.06460436121389078 0.0 20 0.0 0.0 0.0 0.08222373245404281 0.0 21 0.0 0.0 0.0 0.08809685620076015 0.0 22 0.0 0.0 0.0 0.13004774010588402 0.0 23 0.0 0.0 0.0 0.14011595224311377 0.0 24 0.0 0.0 0.0 0.1610913941956757 0.0 25 0.0 0.0 0.0 0.17032058865480296 0.0 26 0.0 0.0 0.0 0.1719986240110079 0.0 27 0.0 0.0 0.0 0.19045701292926243 0.0 28 0.0 0.0 0.0 0.2240177200533615 0.0 29 0.0 0.0 0.0 0.30204636411689195 0.0 30 0.0 0.0 0.0 0.36581170765268023 0.0 31 0.0 0.0 0.0 0.43964526332569825 0.0 32 0.0 0.0 0.0 0.48998632401184694 0.0 33 0.0 0.0 0.0 0.6040927282337839 0.0 34 0.0 0.0 0.0 0.7416916274425902 0.0 35 0.0 0.0 0.0 0.880129544329499 0.0 36 0.0 0.0 0.0 1.1192495825887052 0.0 37 0.0 0.0 0.0 1.5085537852282547 0.0 38 0.0 0.0 0.0 1.9909889501371794 0.0 39 0.0 0.0 0.0 2.5036287514577933 0.0 40 0.0 0.0 0.0 3.0330489063404564 0.0 41 0.0 0.0 0.0 3.6337855638618306 0.0 42 0.0 0.0 0.0 4.281507211356943 0.0 43 0.0 0.0 0.0 5.026554909511943 0.0 44 0.0 0.0 0.0 5.650784062020187 0.0 45 0.0 0.0 0.0 6.260749914000688 0.0 46 0.0 0.0 0.0 6.896725314002366 0.0 47 0.0 0.0 0.0 7.359863072314933 0.0 48 0.0 0.0 0.0 7.855722520073498 0.0 49 0.0 0.0 0.0 8.426254541183182 0.0 50 0.0 0.0 0.0 9.00433772139579 0.0 51 0.0 0.0 0.0 9.466636462030255 0.0 52 0.0 0.0 0.0 9.943198503192463 0.0 53 0.0 0.0 0.0 10.51792561269266 0.0 54 0.0 0.0 0.0 10.977707300292817 0.0 55 0.0 0.0 0.0 11.47356674805138 0.0 56 0.0 0.0 0.0 11.924958258870515 0.0 57 0.0 0.0 0.0 12.304194249372834 0.0 58 0.0 0.0 0.0 12.638962302935722 0.0 59 0.0 0.0 0.0 12.98631562167015 0.0 60 0.0 0.0 0.0 13.40330740768708 0.0 61 0.0 0.0 0.0 13.781704380511297 0.0 62 0.0 0.0 0.0 14.11143832800557 0.0 63 0.0 0.0 0.0 14.486479230117379 0.0 64 0.0 0.0 0.0 14.931997617189793 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATTG 15 0.0022200001 70.0 51 AGTGATT 15 0.0022200001 70.0 17 TGTAGTA 15 0.0022200001 70.0 4 CCGAACG 15 0.0022200001 70.0 35 AGGGAGT 15 0.0022200001 70.0 33 AAAAAGG 485 0.0 56.288666 70 CCCTATC 25 2.382319E-4 55.999996 25 ATGAGAG 25 2.382319E-4 55.999996 5 CTAGACT 20 0.0069368295 52.5 4 CTCACAA 20 0.0069368295 52.5 20 ACACGAC 20 0.0069368295 52.5 28 GACAAGC 20 0.0069368295 52.5 57 GAGAGTA 20 0.0069368295 52.5 7 ATAGAAT 20 0.0069368295 52.5 29 CTTATAA 20 0.0069368295 52.5 1 GTATGCC 20 0.0069368295 52.5 8 CAAGAGC 20 0.0069368295 52.5 32 TGCTACT 20 0.0069368295 52.5 43 GGATTTG 20 0.0069368295 52.5 1 CGTCCGG 20 0.0069368295 52.5 10 >>END_MODULE