FastQCFastQC Report
Wed 25 May 2016
SRR1780049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119187
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3100626.014582127245422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95978.052052656749478No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC35682.99361507546964No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG32162.698280852777568No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT12361.0370258501346625No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT12341.0353478147784574No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT7690.6452045944608054TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC5750.48243516490892463No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC2830.2374420029030012No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2810.23576396754679624No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT2460.20639834881320948No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT2330.1954911189978773No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT1980.16612550026429057No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT1460.12249658100296174No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT1400.11746247493434687No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC1390.11662345725624437No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG1260.10571622744091219No Hit
CACTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC1260.10571622744091219No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC1230.10319917440660474No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA150.002220000170.037
ATGGCAC207.899531E-570.026
GGACCGC150.002220000170.043
AGAGCTA207.899531E-570.020
AGGGGGG38950.064.9679170
AAACACA600.064.1666644
CGCCCAT3050.059.67213432
TGCATGA1200.058.33333255
TGCCGAC308.334275E-658.33333245
CCCATCT3150.057.7777834
CATATAC2250.057.5555571
TAGACCA252.382319E-455.99999631
CCGTCAT1000.055.99999647
ATCGTAT509.695214E-1055.99999639
GCCGACT252.382319E-455.99999646
CATAGAC252.382319E-455.99999629
TCGAACA252.382319E-455.99999631
CCGTCTT48800.055.1536947
TATGCCG49750.054.9447243
GTATGCC49850.054.90471642
Produced by FastQC (version 0.11.5)