FastQCFastQC Report
Wed 25 May 2016
SRR1780048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98392
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4645047.209122692901865No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG73917.511789576388324No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT8520.8659240588665745No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC8230.8364501178957638No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG7690.7815676071225303No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG2670.2713635254898772No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG1860.18903975933002684No Hit
TATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGT1080.10976502154646718No Hit
CTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT1040.10569965037807952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTA150.00221884470.040
CTTCAAT207.8926816E-570.021
TCTAGGT150.00221884470.043
GCCTGGT150.00221884470.021
CTACGAT150.00221884470.037
AGCGCCG150.00221884470.031
AGCAGCA150.00221884470.013
ACCCAGG150.00221884470.06
TGGTCGA207.8926816E-570.049
TTTACAC308.323492E-658.3333322
CTTTACA308.323492E-658.3333321
GGTCGAC252.3802648E-456.00000450
TGACGCA252.3802648E-456.00000413
AGCCGAG200.006933245352.510
CAGCCTC200.006933245352.524
GCAACTC200.006933245352.56
TAGGTGG200.006933245352.545
AGGACCT200.006933245352.525
CCATGTG200.006933245352.532
GCATTGC200.006933245352.545
Produced by FastQC (version 0.11.5)