FastQCFastQC Report
Wed 25 May 2016
SRR1780048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98392
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3139831.911130986259046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1238912.591470851288722No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC34843.540938287665664No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG34223.4779250345556547No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT12191.2389218635661436No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT7270.7388812098544597RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC6290.6392796162289617No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC3110.31608260834214164No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT3100.3150662655500447No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2930.2977884380843971No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG2400.24392227010326042No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2230.22664444263761283No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG2190.22257907146922512No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAACCCATCTCGTATGCCGT1690.1717619318643792No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC1390.14127164810147166No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT1350.137206276933084No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT1190.1209447922595333No Hit
CACTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC1160.11789576388324254No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC1140.1158630782990487No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1110.11281404992275795No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC1040.10569965037807952No Hit
CAAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1040.10569965037807952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT150.002211558970.0580143
ATTGTAG150.002211558970.05801424
ACTTATA353.6434358E-970.0580142
ACGGGGT150.002211558970.05801423
GTCGTTT150.002211558970.05801423
TCTTTAT650.070.0580141
CTCTCTG150.002211558970.05801423
CTAGATT150.00222050669.98678650
CCTAGTT150.00222050669.98678650
GTAATCT150.00222050669.98678649
TAGTTCG150.00222050669.98678652
ATCAACG150.00222050669.98678610
GTTACAG150.00222050669.98678648
TAACCCA353.6725396E-969.98678631
TCTCGTC150.00222050669.98678638
GAAACTT150.00222050669.98678633
CGTAATC150.00222050669.98678648
AGGGGGG37100.066.0252770
ATATACA2450.061.479482
GGTAACC401.0510121E-861.26958529
Produced by FastQC (version 0.11.5)