FastQCFastQC Report
Wed 25 May 2016
SRR1780047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193614
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4980225.72231346906732No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG67303.4759883066307187No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG9350.48291962358093937No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT7130.3682584937039677No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6530.337268999142624No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG2130.11001270569277015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTAG150.00222210370.070
GGCAAAC150.00222210370.064
TTGAGCA150.00222210370.067
CACTCGT150.00222210370.020
TACGCGA150.00222210370.047
TCTCGAC150.00222210370.033
AACGCGC150.00222210370.011
GATGATC150.00222210370.028
CGTAAGG150.00222210370.024
ACGTATC207.9120015E-570.055
GCTCGTT207.9120015E-570.035
TCGACGT207.9120015E-570.052
TCGACGC150.00222210370.035
ATTGAGC150.00222210370.066
CCACACG150.00222210370.035
CGACGTA252.3860564E-456.00000453
GACGTAT252.3860564E-456.00000454
GTAGCGT252.3860564E-456.00000410
ATCCTCG200.00694335252.526
ACACGTT200.00694335252.537
Produced by FastQC (version 0.11.5)