FastQCFastQC Report
Wed 25 May 2016
SRR1780047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193614
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3286516.974495645976013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112685.819827078620348No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC38561.9915915171423555No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG36411.8805458282975405No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT14460.7468468189283833No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT10570.5459315958556716RNA PCR Primer, Index 36 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC7080.3656760358238557No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC3770.19471732416044293No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3220.1663102874792112No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT2810.145134132862293No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG1990.10278182362845661No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATT150.002220444470.01317621
CAGTTAC252.8289069E-670.0131761
CGCATGG150.002220444470.01317619
GGCTTAA150.002220444470.0131761
TGCTCAA150.002220444470.01317628
TCGCATG150.002222720869.99509418
GGTCGCA150.002222720869.99509416
CGTCTTA207.9147474E-569.99509448
CCGCACG150.002222720869.99509436
CTGTGCG150.002222720869.99509453
AGGGGGG40900.062.20835570
TCTTTAT1500.060.678091
ATTAGCG252.3868831E-455.9960810
TTAGCGT252.3868831E-455.9960811
GCGAACT200.00693818352.50988428
GCTATAC200.00693818352.5098841
CTGCCGC200.00693818352.50988433
TAGGCGG200.00693818352.50988426
AGACTTC200.00694527652.4963211
TAGCGTT200.00694527652.4963212
Produced by FastQC (version 0.11.5)