FastQCFastQC Report
Wed 25 May 2016
SRR1780046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405899
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4290210.569624463228537No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG50241.2377463359111502No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG6520.1606310929566222No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT5560.13697988908570852No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC4820.11874875276854588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT150.002224283569.9966715
TTTAGCG200.006946732352.50397527
GACGGTA200.006953506352.4910410
GCCGTAT7900.045.1877354
CGTATCA7850.044.58936756
TGGTCGC8250.042.8517349
TCGCCGT8100.042.78101752
CGCCGTA8100.042.78101753
TCGGTGG8450.042.2517245
CCGTATC8450.041.8374955
GTGGTCG8550.041.75754548
TCTCGGT8500.041.5913843
GTCGCCG8750.041.2031251
CTCGGTG8650.040.87014844
CGGTGGT8850.040.7375546
GTATCAT8300.040.4849957
GATCTCG8750.040.0030341
GGTGGTC8950.039.8912947
AGATCTC9000.039.2807540
TAGATCT8950.039.10428639