FastQCFastQC Report
Wed 25 May 2016
SRR1780046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405899
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT271506.68885609474278Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85252.1002761770785345No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG29040.7154489170951395No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC28100.692290446638203No Hit
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT10380.25572864185425437Illumina PCR Primer Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT7620.18773143072537748Illumina PCR Primer Index 1 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC6130.15102279138406352No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT5160.12712522080616115Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCGA150.002223740670.00098415
ATATCGT850.065.8751737
AGGGGGG36350.061.3275670
ATCGTAT950.058.94093339
ACGATAT950.058.94093334
CCGTCTT44100.053.08944747
GCCGTCT44400.053.04601346
CGTCTTC44700.053.00316248
TATGCCG44950.052.86408243
CGTATGC45750.052.7046341
ACGAATA200.006948425452.5007452
TCGTATG46200.052.4942740
ATGCCGT45200.052.4942744
GTATGCC45400.052.41718742
AAGGGGG42500.052.12372269
TGCCGTC45450.052.0515245
CTCGTAT45550.052.0140839
TTGAAAA46600.051.9749858
GTCTTCT45800.051.80657249
CTTGAAA46650.051.6941957