FastQCFastQC Report
Wed 25 May 2016
SRR1780045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289114
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4593215.887158698644827No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG68872.382105328693872No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG9840.3403501732880455No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC7870.272210961765947No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT7190.24869082783953736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGG200.00694680152.543
GCGTTGG200.00694680152.56
TTCCACG200.00694680152.546
CCGACGT200.00694680152.535
CGACTAC200.00694680152.51
CGTCAGA200.00694680152.52
CGTATCA12050.043.56846256
GTATCAT12250.042.57142657
GCCGTAT12250.042.28571354
CGCCGTA12450.042.16867453
CCGTATC12300.042.11382355
TCGCCGT12400.042.0564552
CGGTGGT12700.041.3385846
GGTGGTC12750.041.1764747
GTGGTCG12850.041.12840348
GGTCGCC12750.040.90196250
GTCGCCG12850.040.8560351
CATTAAA12900.040.69767461
TCGGTGG12850.040.58365645
TCATTAA12900.040.42635760