FastQCFastQC Report
Wed 25 May 2016
SRR1780045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289114
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3021610.451240687064619No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116624.033702968379256No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC36311.255905974805786No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG33171.147298297557365No Hit
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT12450.43062598144676495No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT9900.34242547922272876TruSeq Adapter, Index 25 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC6310.2182530074641837No Hit
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC3480.12036774421162585No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAA150.002223651669.9965458
TAACTCG150.002223651669.9965436
AGGGGGG35950.062.20833670
CTCGTAA553.6379788E-1157.26989739
TCGTAAG452.6951966E-854.44175340
AAGGGGG41600.053.591169
CGAAAAC200.00694340752.50648521
TGCACCG200.00694340752.50648524
GCGGTGT200.00694815952.49740649
CGCGACA200.00694815952.49740613
CTAACGC200.00694815952.49740636
ACTCGTA200.00694815952.49740638
AACTCGT200.00694815952.49740637
TCGGTGT200.00694815952.49740641
CCGTCTT43150.051.17939447
ATGCCGT44800.051.16934644
TATGCCG45150.051.16026743
TGCCGTC44650.051.10609445
GCCGTCT43400.050.88458346
CGTCTTC43500.050.6871548